High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study

被引：86

作者：

Sripalakit, Pattana ^{[1
]}

Neamhom, Penporn

Saraphanchotiwitthaya, Aurasorn

机构：

[1] Naresuan Univ, Dept Pharmaceut Chem & Pharmacognosy, Fac Pharmaceut Sci, Phitsanulok 65000, Thailand

[2] Naresuan Univ, Bioequivalence Test Ctr, Fac Pharmaceut Sci, Phitsanulok 65000, Thailand

[3] Naresuan Univ, Dept Pharmaceut Technol, Fac Pharmaceut Sci, Phitsanulok 65000, Thailand

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 843卷 / 02期

关键词：

pioglitazone; HPLC; pharmacokinetics; quantification; validation; solid-phase extraction; reversed-phase chromatography;

D O I：

10.1016/j.jchromb.2006.05.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method based on high-performance liquid chromatography (HPLC) with ultraviolet detection (269 nm) was developed for the determination of pioglitazone in human plasma. Rosiglitazone was used as an internal standard. Chromatographic separation was achieved with a reversed-phase Apollo C18 column and a mobile phase of methanol-acetonitrile-mixed phosphate buffer (pH 2.6; 10mm) (40:12:48, v/v/v) with a flow rate of 1.2 ml/min. The calibration curve was linear over the range of 50-2000 ng/ml (r(2) > 0.9987) and the lower limit of quantification was 50 ng/ml. The method was validated with excellent sensitivity, accuracy, precision, recovery and stability. The assay has been applied successfully to a pharmacokinetic study with human volunteers. (c) 2006 Elsevier B.V. All rights reserved.

引用

收藏

页码：164 / 169

页数：6

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