Indole-3-acetic acid production via the indole-3-pyruvate pathway by plant growth promoter Rhizobium tropici CIAT 899 is strongly inhibited by ammonium

被引:29
作者
Imada, Eddie Luidy [1 ]
de Paiva Rolla dos Santos, Amanda Alves [2 ]
Martinez de Oliveira, Andre Luiz [3 ]
Hungria, Mariangela [2 ]
Rodrigues, Elisete Pains [1 ]
机构
[1] Univ Estadual Londrina, Dept Biol Geral, Lab Genet Microrganismos, PR-445,Km 380,Campus Univ,POB 6001, BR-86051970 Londrina, PR, Brazil
[2] Embrapa Soja, Lab Biotecnol Solo, POB 231, BR-86001970 Londrina, PR, Brazil
[3] Univ Estadual Londrina, Dept Bioquim & Biotecnol, PR-445,Km 380,Campus Univ,POB 6001, BR-86051970 Londrina, PR, Brazil
关键词
Plant growth regulators; Tryptophan; Indoles; Symbiosis; Nitrogen fixation; Fertilizers; PHASEOLUS-VULGARIS L; AMINO-ACID OXIDASE; INDOLEPYRUVATE FERREDOXIN OXIDOREDUCTASE; BROAD SUBSTRATE-SPECIFICITY; NITROGEN-FIXATION; NODULE DEVELOPMENT; INOCULATION; NODULATION; GENES; BIOSYNTHESIS;
D O I
10.1016/j.resmic.2016.10.010
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Like many rhizobia, Rhizobium tropici produces indole-3-acetic acid (IAA), an important signal molecule required for root hair infection in rhizobia-legume symbioses. However, the IAA biosynthesis pathway and its regulation by R. tropici are still poorly understood. In this study, IAA synthesis and the effects of mineral N in IAA production by R. tropici CIAT 899 were verified by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). Furthermore, expression of genes related to IAA biosynthesis and metabolism were evaluated by RT-qPCR. Results indicated that IAA production by CIAT 899 was 12 times lower in the presence of NH4+. Moreover, it was found that indole-3-pyruvate (IPyA) is the major IAA biosynthesis intermediate. Genes y4wE, lao and iorA were identified by analysis of R. tropici genome in silico and were upregulated by tryptophan, indicating a possible role of these genes in IAA biosynthesis by CIAT 899. In conclusion, we show that IPyA is the major pathway for IAA biosynthesis in CIAT 899 and that its production is strongly inhibited by NH4+. Although present results arose from in vitro experiments, they provide new insight into the role of nitrogen in early events related to legume nodulation. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:283 / 292
页数:10
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