Enantioseparation in capillary electrophoresis using 2-hydroxypropyltrimethylammonium salt of beta-cyclodextrin as a chiral selector

被引:49
作者
Schulte, G [1 ]
Chankvetadze, B [1 ]
Blaschke, G [1 ]
机构
[1] TBILISI STATE UNIV, DEPT CHEM, GE-380028 TBILISI, GEORGIA
关键词
enantiomer separation; chiral selectors; cyclodextrins; 2-hydroxypropyltrimethylammonium beta-cyclodextrin;
D O I
10.1016/S0021-9673(97)00096-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The resolving ability of the 2-hydroxypropyltrimethylammonium salt of beta-cyclodextrin (TMA-beta-CD) as a chiral selector in capillary electrophoresis (CE) is reported in this work in continuation of our research on this subject [Chem. Sec. Rev., 25 (1996) 141; J. Chromatogr. A, 732 (1996) 143, 183]. The composition of the chiral selector and the effect of its addition to the background electrolyte on the EOF in a fused-silica and a polyacrylamide-coated capillary were studied. The enantioseparations of neutral chiral analytes are reported first using a positively charged cyclodextrin (CD) derivative. The carrier ability of a positively charged CD derivative is used for the migration of neutral analytes in a polyacrylamide-coated capillary. Several general advantages of this mode of electrophoretic separations are illustrated together with selected possibilities for the reversal of the enantiomer migration order for neutral and anionic analytes.
引用
收藏
页码:259 / 266
页数:8
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