An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

被引：7

作者：

Cai, Kai ^{[1
,2
]}

Tan, Ying ^{[1
,2
]}

Tan, Chunyan ^{[1
,2
]}

Wu, Jiatao ^{[1
,2
]}

Wu, Pan ^{[1
,2
]}

Liang, Jiamei ^{[1
,2
]}

Liu, Shuwen ^{[1
,2
]}

Zhang, Bibo ^{[1
,2
]}

Jiang, Yuyang ^{[2
,3
]}

机构：

[1] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

[2] Tsinghua Univ, Grad Sch Shenzhen, State Key Lab Breeding Base, Shenzhen Key Lab Chem Biol, Shenzhen 518055, Peoples R China

[3] Tsinghua Univ, Sch Pharmaceut Sci, Beijing 100084, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 30期

基金：

中国国家自然科学基金;

关键词：

MOLECULAR WIRE APPROACH; NONSPECIFIC INTERACTIONS; CHEMICAL SENSORS; FUSED PROTEINS; POLYMERS; CELL; NANOPARTICLES; PHOTOPHYSICS; RECOGNITION; DIAGNOSIS;

D O I：

10.1039/c7cc00850c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The iminodiacetate-Ni2+-hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization of hexahistidine-tagged (His-tagged) proteins using PPEIDA was demonstrated.

引用

页码：4191 / 4194

页数：4

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