Propagation of human astrovirus in the PLC/PRF/5 hepatoma cell line

被引：10
|
作者：
Taylor, MB [1 ]
Grabow, WOK [1 ]
Cubitt, WD [1 ]
机构：
[1] INST CHILD HLTH,DEPT VIROL,LONDON WC1N 1EH,ENGLAND
来源：
JOURNAL OF VIROLOGICAL METHODS | 1997年 / 67卷 / 01期
关键词：
human astroviruses; propagation; PLC/PRF/5 cell line;
D O I：
10.1016/S0166-0934(97)00071-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Astroviruses are associated with gastroenteritis in humans and many diseases in animals. Human astroviruses (HAstVs) cannot be propagated readily or isolated in conventional cell cultures. The presence of trypsin supports HAstV growth in selected cell cultures such as the continuous colonic carcinoma cell line (CaCo-2). This study reports on the propagation of cell culture adapted reference strains of HAstV, and the direct isolation of HAstV from stool specimens in the human hepatoma cell line PLC/PRF/5. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：13 / 18
页数：6
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