Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture
被引:18
作者:
Bettinger, Christopher J.
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机构:
MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA
Ctr Biomed Engn, Charles Stark Draper Lab, Cambridge, MA USAMIT, Dept Chem Engn, Cambridge, MA 02139 USA
Bettinger, Christopher J.
[2
,3
]
Kulig, Katherine M.
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机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Boston, MA USAMIT, Dept Chem Engn, Cambridge, MA 02139 USA
Kulig, Katherine M.
[4
]
Vacanti, Joseph P.
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机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Boston, MA USAMIT, Dept Chem Engn, Cambridge, MA 02139 USA
Vacanti, Joseph P.
[4
]
Langer, Robert
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机构:
MIT, Dept Chem Engn, Cambridge, MA 02139 USAMIT, Dept Chem Engn, Cambridge, MA 02139 USA
Langer, Robert
[1
]
Borenstein, Jeffrey T.
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机构:
Ctr Biomed Engn, Charles Stark Draper Lab, Cambridge, MA USAMIT, Dept Chem Engn, Cambridge, MA 02139 USA
Borenstein, Jeffrey T.
[3
]
机构:
[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[2] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA
[3] Ctr Biomed Engn, Charles Stark Draper Lab, Cambridge, MA USA
[4] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Boston, MA USA
Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological characteristics of collagen. We found that elastomeric poly(ester amide) substrates modified with replica-molded nanotopographic features enhanced initial attachment, spreading, and adhesion of primary rat hepatocytes. Further, hepatocytes cultured on nanotopographic substrates also demonstrated reduced albumin secretion and urea synthesis, which is indicative of strongly adherent hepatocytes. These results suggest that these engineered substrates can function as synthetic collagen analogs for in vitro cell culture.