Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon

被引：47
作者：
Dang, Hongyu [1 ]
Yu, Najiaowa [1 ]
Mou, Anqi [1 ]
Zhang, Lei [1 ]
Guo, Bing [2 ]
Liu, Yang [1 ]
机构：
[1] Univ Alberta, Dept Civil & Environm Engn, Edmonton, AB T6G 1H9, Canada
[2] Univ Surrey, Ctr Environm Hlth & Engn CEHE, Dept Civil & Environm Engn, Guildford GU2 7XH, Surrey, England
来源：
BIORESOURCE TECHNOLOGY | 2022年 / 352卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
Direct interspecies electron transfer (DIET); Microbial community analysis; Shotgun sequencing; COMMUNITIES; BIOGAS; GENE;
D O I：
10.1016/j.biortech.2022.127113
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The addition of granular activated carbon (GAC) enhanced the performance of up-flow anaerobic sludge blanket (UASB) reactor treating blackwater at 35 degrees C. DNA were extracted from the sludge and biofilms attached to GAC and submitted for shotgun sequencing. In addition, the acyl-homoserine lactones (AHLs) were quantified. Diverse partners for direct interspecies electron transfer (DIET) were enriched in the sludge or biofilm (GAC-biofilm) of GAC amended UASB. Pedosphaera parvula, Syntrophus aciditrophicus and Syntrophorhabus aromaticivorans were dominant syntrophs. The analysis for type IV pilus assembly genes further suggested DIET may be functioned through GAC for GAC-biofilm, while through conductive pili for sludge aggregates. AHLs quantification and the analysis for quorum sensing (QS) related genes indicated higher QS activity at the population level was induced by GAC. Overall, the work illustrated the different DIET patterns, and suggested that QS played an important role in controlling the performance in GAC amended USAB.
引用
收藏
页数：9
相关论文
共 50 条
[21]   Syntrophy Goes Electric: Direct Interspecies Electron Transfer [J].
Lovley, Derek R. .
ANNUAL REVIEW OF MICROBIOLOGY, VOL 71, 2017, 71 :643-664
[22]   Reach out and touch someone: potential impact of DIET (direct interspecies energy transfer) on anaerobic biogeochemistry, bioremediation, and bioenergy [J].
Lovley, Derek R. .
REVIEWS IN ENVIRONMENTAL SCIENCE AND BIO-TECHNOLOGY, 2011, 10 (02) :101-105
[23]  
Maestrojuan G.M., 1990, TRANSFER METHANOGENI
[24]   Review on research achievements of biogas from anaerobic digestion [J].
Mao, Chunlan ;
Feng, Yongzhong ;
Wang, Xiaojiao ;
Ren, Guangxin .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2015, 45 :540-555
[25]   MetaGene: prokaryotic gene finding from environmental genome shotgun sequences [J].
Noguchi, Hideki ;
Park, Jungho ;
Takagi, Toshihisa .
NUCLEIC ACIDS RESEARCH, 2006, 34 (19) :5623-5630
[26]   Let's chat: Communication between electroactive microorganisms [J].
Paquete, Catarina M. ;
Rosenbaum, Miriam A. ;
Baneras, Lluis ;
Rotaru, Amelia-Elena ;
Puig, Sebastia .
BIORESOURCE TECHNOLOGY, 2022, 347
[27]  
Pelletier E., 2008, GENOME SEQUENCE RECO
[28]   Cyclic voltammetry of biofilms of wild type and mutant Geobacter sulfurreducens on fuel cell anodes indicates possible roles of OmcB, OmcZ, type IV pili, and protons in extracellular electron transfer [J].
Richter, Hanno ;
Nevin, Kelly P. ;
Jia, Hongfei ;
Lowy, Daniel A. ;
Lovley, Derek R. ;
Tender, Leonard M. .
ENERGY & ENVIRONMENTAL SCIENCE, 2009, 2 (05) :506-516
[29]   Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri [J].
Rotaru, Amelia-Elena ;
Shrestha, Pravin Malla ;
Liu, Fanghua ;
Markovaite, Beatrice ;
Chen, Shanshan ;
Nevin, Kelly P. ;
Lovley, Derek R. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (15) :4599-4605
[30]   A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane [J].
Rotaru, Amelia-Elena ;
Shrestha, Pravin Malta ;
Liu, Fanghua ;
Shrestha, Minita ;
Shrestha, Devesh ;
Embree, Mallory ;
Zengler, Karsten ;
Wardman, Colin ;
Nevin, Kelly P. ;
Lovley, Derek R. .
ENERGY & ENVIRONMENTAL SCIENCE, 2014, 7 (01) :408-415
12345