Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon

被引：47

作者：

Dang, Hongyu ^{[1
]}

Yu, Najiaowa ^{[1
]}

Mou, Anqi ^{[1
]}

Zhang, Lei ^{[1
]}

Guo, Bing ^{[2
]}

Liu, Yang ^{[1
]}

机构：

[1] Univ Alberta, Dept Civil & Environm Engn, Edmonton, AB T6G 1H9, Canada

[2] Univ Surrey, Ctr Environm Hlth & Engn CEHE, Dept Civil & Environm Engn, Guildford GU2 7XH, Surrey, England

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 352卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Direct interspecies electron transfer (DIET); Microbial community analysis; Shotgun sequencing; COMMUNITIES; BIOGAS; GENE;

D O I：

10.1016/j.biortech.2022.127113

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The addition of granular activated carbon (GAC) enhanced the performance of up-flow anaerobic sludge blanket (UASB) reactor treating blackwater at 35 degrees C. DNA were extracted from the sludge and biofilms attached to GAC and submitted for shotgun sequencing. In addition, the acyl-homoserine lactones (AHLs) were quantified. Diverse partners for direct interspecies electron transfer (DIET) were enriched in the sludge or biofilm (GAC-biofilm) of GAC amended UASB. Pedosphaera parvula, Syntrophus aciditrophicus and Syntrophorhabus aromaticivorans were dominant syntrophs. The analysis for type IV pilus assembly genes further suggested DIET may be functioned through GAC for GAC-biofilm, while through conductive pili for sludge aggregates. AHLs quantification and the analysis for quorum sensing (QS) related genes indicated higher QS activity at the population level was induced by GAC. Overall, the work illustrated the different DIET patterns, and suggested that QS played an important role in controlling the performance in GAC amended USAB.

引用

页数：9

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