miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN

被引：63

作者：

Liu Meng Xin ^{[1
]}

Liao Juan ^{[1
,2
]}

Xie Ming ^{[3
]}

Gao Zhi Kui ^{[1
]}

Wang Xiang Hu ^{[1
]}

Zhang Ying ^{[1
]}

Shang Mu He ^{[1
]}

Yin Li Hong ^{[1
]}

Pu Yue Pu ^{[1
]}

Liu Ran ^{[1
]}

机构：

[1] Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn, Minist Educ, Nanjing 210009, Jiangsu, Peoples R China

[2] Hangzhou First Peoples Hosp, Hangzhou 310000, Zhejiang, Peoples R China

[3] North China Petr Bur, Gen Hosp, Renqiu 062552, Hebei, Peoples R China

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2018年 / 31卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Exosomes; miR-93-5p; Esophageal cancer; Plasma biomarker; MICRORNA CLUSTER; EXPRESSION; BIOMARKER; PLASMA; RECURRENCE; TUMOR; LIVER; CYCLE;

D O I：

10.3967/bes2018.023

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Objective To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes. Methods The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting. Results Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin Dl. Conclusion Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.

引用

页码：171 / 185

页数：15

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