Hepatoprotective effects of daidzein against 7,12-dimetylbenz[a]anthracene-induced oxidative stress in mice

被引:24
作者
Choi, Eun Jeong [1 ]
Kim, Gun-Hee [1 ]
机构
[1] Duksung Womens Univ, Plant Resources Res Inst, Seoul 132714, South Korea
关键词
antioxidant; apoptosis; daidzein; 7,12-dimethylbenz[a]anthracene; oxidative stress; in vivo; BREAST-CANCER; DOWN-REGULATION; IN-VITRO; ANTIOXIDANT; APOPTOSIS; GENISTEIN; GLUTATHIONE; TOXICITY; CELLS; ISOFLAVONES;
D O I
10.3892/ijmm_00000177
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We investigated the effects of daidzein on the antioxidant defence system in mice with 7,12-dimethylbenz[a]-anthracene (DMBA)-induced oxidative stress. Daidzein was administered orally at 5 and 25 mg/kg body weight for 5 weeks. Subsequently, mice pretreated with daidzein received DMBA intragastrically twice a week for 2 weeks. As controls, mice were given vehicle or DMBA alone. In the DMBA group, biomarkers of oxidative stress (thiobarbituric acid reactive substances value, carbonyl content) were significantly increased. However, the rise in oxidative damage was significantly reduced by daidzein at the higher dose. In addition, several antioxidant enzymes were downregulated in the DMBA-treated mice. Catalase and superoxide dismutase activity was increased by daidzein in a dose-dependent manner. Although the reduced/oxidized glutathione ratio was unaffected, glutathione peroxidase and reductase were activated by daidzein, and the effect was significant at the higher dose. Further, in the DMBA-treated mice, apoptosis was induced by a decrease in Bcl-2 and an increase in Bax. These changes were restored to their normal values in the daidzein-treated mice. Upregulation of caspase-3 was also decreased by daidzein. These results suggest that daidzein exerts a hepatoprotective effect on mice with DMBA-induced oxidative stress through its antioxidant activity and the reduction of apoptosis.
引用
收藏
页码:659 / 664
页数:6
相关论文
共 46 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   Protective effect of Operculina turpethum against 7,12-dimethyl benz(a)anthracene induced oxidative stress with reference to breast cancer in experimental rats [J].
Anbuselvam, C. ;
Vijayavel, K. ;
Balasubramanian, M. P. .
CHEMICO-BIOLOGICAL INTERACTIONS, 2007, 168 (03) :229-236
[3]   Effects of soy isoflavones on atherosclerosis: potential mechanisms [J].
Anthony, MS ;
Clarkson, TB ;
Williams, JK .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1998, 68 (06) :1390S-1393S
[4]   Structure-activity relationships for antioxidant activities of a series of flavonoids in a liposomal system [J].
Arora, A ;
Nair, MG ;
Strasburg, GM .
FREE RADICAL BIOLOGY AND MEDICINE, 1998, 24 (09) :1355-1363
[5]   Drug discovery from medicinal plants [J].
Balunas, MJ ;
Kinghorn, AD .
LIFE SCIENCES, 2005, 78 (05) :431-441
[6]  
CARLBERG I, 1985, METHOD ENZYMOL, V113, P484
[7]   The prooxidant, rather than antioxidant, acts of daidzein in vivo and in vitro: Daidzein suppresses glutathione metabolism [J].
Choi, Eun Jeong .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2006, 542 (1-3) :162-169
[8]  
Cline J M, 1998, Cancer Treat Res, V94, P107
[9]   Antioxidant intervention as a route to cancer prevention [J].
Collins, AR .
EUROPEAN JOURNAL OF CANCER, 2005, 41 (13) :1923-1930
[10]  
Das Undurti, 2002, Med Sci Monit, V8, pRA79