First report of Bursaphelenchus xylophilus in Portugal and in Europe

被引：561
作者：
Mota, MM [1 ]
Braasch, H
Bravo, MA
Penas, AC
Burgermeister, W
Metge, K
Sousa, E
机构：
[1] Univ Evora, ICAM, Dept Biol, P-7000 Evora, Portugal
[2] Fed Biol Res Ctr Agr & Forestry, Dept Natl & Int Plant Hlth, Kleinmachnow Branch, D-14532 Kleinmachnow, Germany
[3] INIA, Estacao Agron Nacl, Dept Fitopatol, P-2780 Oeiras, Portugal
[4] Fed Biol Res Ctr Agr & Forestry, Inst Plant Virol Microbiol & Biosafety, D-38104 Braunschweig, Germany
[5] INIA, Estacao Florestal Nacl, Dept Proteccao Florestal, P-2780 Oeiras, Portugal
来源：
NEMATOLOGY | 1999年 / 1卷
关键词：
ITS-RFLP; morphology; morphometrics; pinewood nematode (PWN); SEM;
D O I：
10.1163/156854199508757
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
A survey of aphelenchid nematodes (Nematoda: Aphelenchida) associated with maritime pine, Pinus pinaster, was conducted in Portugal in 1996 and 1999. A Bursaphelenchus species has been identified for the first time in the Iberian Peninsula. B. xylophilus is reported for the first time in Europe. It was found in very high numbers - up to 38 000 per 10 g of pine wood inside a few declining tr ces infested with curculionid, cerambycid and scolytid beetles. Morphological observations, including shape of spicules. bursa, vulva, female tail end and stylet as well as morphometrics, were in accordance with the species description. Species-specific DNA fragment patterns were obtained using ITS-RFLP analysis, with five different restriction enzymes The importance and implications of this finding are discussed.
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页码：727 / 734
页数：8
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