Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis

被引:56
作者
Chen, Yangzong [1 ]
Bao, Chunchun [1 ]
Zhang, Xiuxing [1 ]
Lin, Xinshi [1 ]
Huang, Hongou [1 ]
Wang, Zhiqiang [1 ]
机构
[1] Wenzhou Med Univ, Affiliated Hosp 1, Dept Radiol, Div PET CT, Wenzhou, Peoples R China
关键词
CPEB3; glioma; HCG11; miR-496; proliferation; COLORECTAL-CANCER; CELLS; PROLIFERATION; KNOCKDOWN; MICRORNAS; PROSTATE; BLADDER; GROWTH;
D O I
10.1111/cpr.12615
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Objectives It has been widely reported that long non-coding RNAs (lncRNAs) can participate in multiple biological processes of human cancers. lncRNA HLA complex group 11 (HCG11) has been reported in human cancers as a tumour suppressor. This study focused on investigating the function and mechanism of HCG11 in glioma. Materials and methods Based on The Cancer Genome Atlas (TCGA) data set and qRT-PCR analysis, the expression pattern of HCG11 was identified in glioma samples. The mechanism associated with HCG11 downregulation was determined by mechanism experiments. Gain-of-function assays were conducted for the identification of HCG11 function in glioma progression. Mechanism investigation based on the luciferase reporter assay, RIP assay and pull-down assay was used to explore the downstream molecular mechanism of HCG11. The role of molecular pathway in the progression of glioma was analysed in accordance with the rescue assays. Results HCG11 was expressed at low level in glioma samples compared with normal samples. FOXP1 could bind with HCG11 and transcriptionally inactivated HCG11. Overexpression of HCG11 efficiently suppressed cell proliferation, induced cell cycle arrest and promoted cell apoptosis. HCG11 was predominantly enriched in the cytoplasm of glioma cells and acted as a competing endogenous RNAs (ceRNAs) by sponging micro-496 to upregulate cytoplasmic polyadenylation element binding protein 3 (CPEB3). CEPB3 and miR-496 involved in HCG11-mediated glioma progression. Conclusions HCG11 inhibited glioma progression by regulating miR-496/CPEB3 axis.
引用
收藏
页数:13
相关论文
共 34 条
[1]  
Acunzo Mario, 2015, Adv Biol Regul, V57, P1, DOI 10.1016/j.jbior.2014.09.013
[2]  
[Anonymous], 2018, ONCOL RES
[3]   MicroRNAs involved in chemo- and radioresistance of high-grade gliomas [J].
Besse, Andrej ;
Sana, Jiri ;
Fadrus, Pavel ;
Slaby, Ondrej .
TUMOR BIOLOGY, 2013, 34 (04) :1969-1978
[4]   Translational control of cell growth and malignancy by the CPEBs [J].
D'Ambrogio, Andrea ;
Nagaoka, Kentaro ;
Richter, Joel D. .
NATURE REVIEWS CANCER, 2013, 13 (04) :283-290
[5]   Knockdown of Long Noncoding RNA HOXA-AS2 Suppresses Chemoresistance of Acute Myeloid Leukemia via the miR-520c-3p/S100A4 Axis [J].
Dong, Xiaoya ;
Fang, Zhigang ;
Yu, Mingxue ;
Zhang, Ling ;
Xiao, Ruozhi ;
Li, Xudong ;
Pan, Guangjin ;
Liu, Jiajun .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 51 (02) :886-896
[6]   RETRACTED: Long Noncoding RNA FER1L4 Suppresses Tumorigenesis by Regulating the Expression of PTEN Targeting miR-18a-5p in Osteosarcoma (Retracted article. See vol. 55, pg. 515, 2021) [J].
Fei, Dan ;
Zhang, Xiaona ;
Liu, Jinxiang ;
Tan, Long ;
Xing, Jie ;
Zhao, Dongxu ;
Zhang, Yang .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 51 (03) :1364-1375
[7]   The role of CXCR4 in highly malignant human gliomas biology: Current knowledge and future directions [J].
Gagliardi, Filippo ;
Narayanan, Ashwin ;
Reni, Michele ;
Franzin, Alberto ;
Mazza, Elena ;
Boari, Nicola ;
Bailo, Michele ;
Zordan, Paola ;
Mortini, Pietro .
GLIA, 2014, 62 (07) :1015-1023
[8]   lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3′ UTRs via Alu elements [J].
Gong, Chenguang ;
Maquat, Lynne E. .
NATURE, 2011, 470 (7333) :284-+
[9]   A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature [J].
Gonzalez, Inma ;
Munita, Roberto ;
Agirre, Eneritz ;
Dittmer, Travis A. ;
Gysling, Katia ;
Misteli, Tom ;
Luco, Reini F. .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2015, 22 (05) :370-U111