Structural and functional peculiarities of the ATP-dependent Lon protease from Escherichia coli

被引：0

作者：

Rotanova, TV ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 1999年 / 25卷 / 12期

关键词：

active site; ATP-dependent proteolysis; Escherichia coli; Lon protease; interdomain interactions; site-directed mutagenesis;

D O I：

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学科分类号：

摘要：

Enzymic and structural peculiarities of the ATP-dependent Lon protease from Escherichia coli and its mutant and modified forms were studied. Amino acid residues important for the function of proteolytic and ATPase sites and for the transmition of the interdomain signals of the activity coupling were found. It was shown that the protein substrates are hydrolyzed only by the full-size enzyme, whereas the isolated proteolytic domain displays a peptide-hydrolyzing activity.

引用

页码：883 / 891

页数：9

共 35 条

[1]

AMERIK AY, 1990, BIOORG KHIM+, V16, P869

[2] CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR A HUMAN HOMOLOG OF EUBACTERIAL ATP-DEPENDENT LON PROTEASES [J].

AMERIK, AY ;

PETUKHOVA, GV ;

GRIGORENKO, VG ;

LYKOV, IP ;

YAROVOI, SV ;

LIPKIN, VM ;

GORBALENYA, AE .

FEBS LETTERS, 1994, 340 (1-2) :25-28

[3] SITE-DIRECTED MUTAGENESIS OF LA PROTEASE - A CATALYTICALLY ACTIVE SERINE RESIDUE [J].

AMERIK, AY ;

ANTONOV, VK ;

GORBALENYA, AE ;

KOTOVA, SA ;

ROTANOVA, TV ;

SHIMBAREVICH, EV .

FEBS LETTERS, 1991, 287 (1-2) :211-214

[4]

AMERIK AY, 1988, BIOORG KHIM+, V14, P408

[5]

ANTONOV VK, 1968, KHIMIYA PROTEOLIZA

[6] LOCATION OF ENZYMATIC AND DNA-BINDING DOMAINS ON ESCHERICHIA-COLI PROTEASE LA [J].

BAKER, ME .

FEBS LETTERS, 1989, 244 (01) :31-33

[7] ATP HYDROLYSIS-DEPENDENT PROTEASE ACTIVITY OF THE ION (CAPR) PROTEIN OF ESCHERICHIA-COLI K-12 [J].

CHARETTE, MF ;

HENDERSON, GW ;

MARKOVITZ, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (08) :4728-4732

[8]

CHIN DT, 1988, J BIOL CHEM, V263, P11718

[9]

Fisher H., 1993, J BIOL CHEM, V268, P22502

[10]

GOLDBERG AL, 1994, METHOD ENZYMOL, V244, P350

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