Epigenetic changes of mesenchymal stem cells in three-dimensional (3D) spheroids

被引：102
作者：
Guo, Ling [1 ]
Zhou, Ying [1 ,2 ]
Wang, Shan [1 ,2 ]
Wu, Yaojiong [1 ]
机构：
[1] Tsinghua Univ, Shenzhen Key Lab Hlth Sci & Technol, Grad Sch Shenzhen, Shenzhen 518057, Peoples R China
[2] Tsinghua Univ, Sch Life Sci, Shenzhen 518057, Peoples R China
来源：
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2014年 / 18卷 / 10期
基金：
中国博士后科学基金;
关键词：
mesenchymal stem cells; miRNA; histone acetylation; 3D culture; multi-potency; MARROW STROMAL CELLS; DIFFERENTIATION CAPACITY; TRANSPLANTATION; PROLIFERATION; OCT4; AGE;
D O I：
10.1111/jcmm.12336
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Mesenchymal stem cells (MSCs) hold profound promise in tissue repair/regeneration. However, MSCs undergo remarkable spontaneous differentiation and aging during monolayer culture expansion. In this study, we found that 2-3days of three-dimensional (3D) spheroid culture of human MSCs (hMSCs) that had been expanded in monolayer for six passages increased their clonogenicity and differentiation potency to neuronal cells. Moreover, in accordance with these changes, the expression levels of miRNA which were involved in stem cell potency were changed and levels of histone H3 acetylation in K9 in promoter regions of Oct4, Sox2 and Nanog were elevated. Our results indicate that spheroid culture increases their multi-potency and changes the epigenetic status of pluripotent genes in hMSCs.
引用
收藏
页码：2009 / 2019
页数：11
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