Chitosan nanoparticles enhance developmental competence of in vitro-matured porcine oocytes

被引:16
|
作者
Roy, Pantu Kumar [1 ]
Qamar, Ahmad Yar [1 ,2 ]
Fang, Xun [1 ]
Kim, Ghangyong [1 ]
Bang, Seonggyu [1 ]
De Zoysa, Mahanama [1 ]
Shin, Sang Tae [1 ]
Cho, Jongki [1 ]
机构
[1] Chungnam Natl Univ, Coll Vet Med, Daejeon 34134, South Korea
[2] Univ Vet & Anim Sci, Dept Clin Sci, Coll Vet & Anim Sci, Subcampus, Lahore 35200, Pakistan
关键词
chitosan nanoparticles; development; embryo; oxidative damage; porcine; BOVINE EMBRYONIC-DEVELOPMENT; IN-VITRO; OXIDATIVE STRESS; REACTIVE OXYGEN; DNA-DAMAGE; PREIMPLANTATION EMBRYO; TRANSCRIPTION FACTOR; GLUTATHIONE CONTENT; MEIOTIC RESUMPTION; MATURATION;
D O I
10.1111/rda.13871
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus-oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 mu g/ml) during in vitro maturation (IVM). Oocytes treated with 25 mu g/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 mu g/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress.
引用
收藏
页码:342 / 350
页数:9
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