Major triterpenoids in Chinese hawthorn "Crataegus pinnatifida" and their effects on cell proliferation and apoptosis induction in MDA-MB-231 cancer cells

被引：42
作者：
Wen, Lingrong [1 ,2 ]
Guo, Ruixue [1 ,2 ]
You, Lijun [1 ,2 ]
Abbasi, Arshad Mehmood [1 ,4 ]
Li, Tong [3 ]
Fu, Xiong [1 ,2 ]
Liu, Rui Hai [3 ]
机构：
[1] South China Univ Technol, Sch Food Sci & Technol, Guangzhou 510640, Guangdong, Peoples R China
[2] South China Univ Technol, Ctr Guangdong Food Green Proc & Nutr Regulat Engn, Guangzhou 510640, Guangdong, Peoples R China
[3] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA
[4] COMSATS Inst Informat Technol, Dept Environm Sci, Abbottabad, Pakistan
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2017年 / 100卷
关键词：
Crataegus pinnatifida; MDA-MB-231; Ursolic acid; Apoptosis; Cell cycle; Semi-preparative HPLC; ACID INDUCES APOPTOSIS; URSOLIC ACID; ANTIOXIDANT ACTIVITY; CYCLE ARREST; CASPASE-3; ACTIVATION; FRUIT; PATHWAY; POLYPHENOLS; EXTRACT; PEELS;
D O I：
10.1016/j.fct.2016.12.032
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The cytotoxicity and antiproliferative effect of phytochemicals presenting in the fruits of Chinese hawthorn (Crataegus pinnatifida) were evaluated. Shanlihong (Crataegus pinnatifida Bge. var. major N.E.Br.) variety possessed significant levels of flavonoids and triterpenoids, and showed potent antiproliferative effect against HepG(2), MCF-7 and MDA-MB- 231 human cancer cells lines. Triterpenoids-enriched fraction (S9) prepared by Semi-preparative HPLC, and its predominant ingredient ursolic acid (UA) demonstrated remarkably antiproliferative activities for all the tested cancer cell lines. DNA flow cytometric analysis showed that S9 fraction and UA significantly induced G1 arrest in MDA-MB-231 cells in a dose dependent manner. Western blotting analysis revealed that S9 fraction and UA significantly induced PCNA, CDK4, and Cyclin D1 downregulation in MDA-MB-231 cells, followed by p21(Waf1/Cip1) up-regulation. Additionally, flow cytometer and DNA ladder assays indicated that S9 fraction and UA significantly induced MDA-MB-231 cells apoptosis. Mitochondrial death pathway was involved in this apoptosis as significantly induced caspase-9 and caspase-3 activation. These results suggested that triterpenoids-enriched fraction and UA exhibited antiproliferative activity through the cell cycle arrest and apoptosis induction, and was majorly responsible for the potent anticancer activity of Chinese hawthorn. (C) 2016 Elsevier Ltd. All rights reserved.
引用
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页码：149 / 160
页数：12
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