Immobilization of RGD Peptide onto the Surface of Apatite-Wollastonite Ceramic for Enhanced Osteoblast Adhesion and Bone Regeneration

被引：9
作者：
Zhang Xiang [1 ]
Gu Jianwen [1 ,2 ]
Zhang Yue [3 ]
Tan Yanfei [1 ]
Zhou Jiabei [1 ]
Zhou Dali [1 ]
机构：
[1] Sichuan Univ, Coll Mat Sci & Engn, Chengdu 610064, Peoples R China
[2] Chengdu PLA, Mil Gen Hosp, Chengdu 610083, Peoples R China
[3] Gannan Med Univ, Affiliated Hosp 1, Ganzhou 341000, Peoples R China
来源：
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION | 2014年 / 29卷 / 03期
基金：
高等学校博士学科点专项科研基金;
关键词：
apatite-wollastonite ceramic; surface modification; RGD peptide; ostcoinduction; bone regeneration; MESOPOROUS CAO-MO-SIO2-P2O5 M; DIFFERENTIATION; ATTACHMENT; ALLOY; CELLS; MEPE; ZN; MG;
D O I：
10.1007/s11595-014-0969-5
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The arginine-glycine-aspartic (RGD) acid peptide was grafted to the surface of apatite-wollastonite (AW) ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalently immobilized onto the surface of AW ceramic via the synthetic Cross linker AAPTS-E and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). The modified surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The chemical analysis indicated that ROD peptide had been immobilized onto the AW surface successfully. The growth of osteoblast-like cells (MG63) showed that modifying the AW surface with ROD peptide enhanced the cell adhesion and proliferation. And the histological evaluation of RGD-AW showed that the bone regeneration and remodeling process were significantly enhanced compared to the original AW ceramics after 2, 4 and 8 weeks implantation in rabbit's femoral condyles.
引用
收藏
页码：626 / 634
页数：9
相关论文
共 43 条
[1]   Surface immobilization of MEPE peptide onto HA/ß-TCP ceramic particles enhances bone regeneration and remodeling [J].
Acharya, Bodhraj ;
Chun, So-Young ;
Kim, Shin-Yoon ;
Moon, Cheil ;
Shin, Hong-In ;
Park, Eui Kyun .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2012, 100B (03) :841-849
[2]   RGD peptide immobilized on TiO2 nanotubes for increased bone marrow stromal cells adhesion and osteogenic gene expression [J].
Cao, Xin ;
Yu, Wei-qiang ;
Qiu, Jing ;
Zhao, Yan-fang ;
Zhang, Yi-lin ;
Zhang, Fu-qiang .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2012, 23 (02) :527-536
[3]   Selective adhesion and growth of vascular endothelial cells on bioactive peptide nanofiber functionalized stainless steel surface [J].
Ceylan, Hakan ;
Tekinay, Ayse B. ;
Guler, Mustafa O. .
BIOMATERIALS, 2011, 32 (34) :8797-8805
[4]   Fabrication and characterization of sol-gel derived 45S5 Bioglass®-ceramic scaffolds [J].
Chen, Qi-Zhi ;
Thouas, George A. .
ACTA BIOMATERIALIA, 2011, 7 (10) :3616-3626
[5]   Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes [J].
De Bartolo, L ;
Morelli, S ;
Lopez, LC ;
Giorno, L ;
Campana, C ;
Salerno, S ;
Rende, M ;
Favia, P ;
Detomaso, L ;
Gristina, R ;
d'Agostino, R ;
Drioll, E .
BIOMATERIALS, 2005, 26 (21) :4432-4441
[6]   The chemical composition of synthetic bone substitutes influences tissue reactions in vivo: histological and histomorphometrical analysis of the cellular inflammatory response to hydroxyapatite, beta-tricalcium phosphate and biphasic calcium phosphate ceramics [J].
Ghanaati, Shahram ;
Barbeck, Mike ;
Detsch, Rainer ;
Deisinger, Ulrike ;
Hilbig, Ulrike ;
Rausch, Vera ;
Sader, Robert ;
Unger, Ronald E. ;
Ziegler, Guenter ;
Kirkpatrick, Charles James .
BIOMEDICAL MATERIALS, 2012, 7 (01)
[7]   Influence of strontium on structure, sintering and biodegradation behaviour of CaO-MgO-SrO-SiO2-P2O5-CaF2 glasses [J].
Goel, Ashutosh ;
Rajagopal, Raghu Raman ;
Ferreira, Jose M. F. .
ACTA BIOMATERIALIA, 2011, 7 (11) :4071-4080
[8]   BIOMECHANICALLY INDUCED TISSUE-REACTIONS AFTER AI2O3-CERAMIC HIP-JOINT REPLACEMENT - EXPERIMENTAL AND EARLY CLINICAL RESULTS [J].
GRISS, P ;
SILBER, R ;
MERKLE, B ;
HAEHNER, K ;
HEIMKE, G ;
KREMPIEN, B .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1976, 10 (04) :519-528
[9]   Microporosity enhances bioactivity of synthetic bone graft substitutes [J].
Hing, KA ;
Annaz, B ;
Saeed, S ;
Revell, PA ;
Buckland, T .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2005, 16 (05) :467-475
[10]   Comparative performance of three ceramic bone graft substitutes [J].
Hing, Karin A. ;
Wilson, Lester F. ;
Buckland, Thomas .
SPINE JOURNAL, 2007, 7 (04) :475-490
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