A novel enzyme support derived from aminated silica gel and polysuccinimide: preparation and application for the immobilization of porcine pancreatic lipase

被引：28

作者：

Bai, ZW ^{[1
]}

Zhou, YK

机构：

[1] Wuhan Inst Chem Technol, Dept Chem & Pharmaceut Engn, Wuhan 430073, Hubei Province, Peoples R China

[2] Huazhong Univ Sci & Technol, Dept Publ Hlth, Tongji Med Coll, Wuhan 430070, Hubei Province, Peoples R China

来源：

REACTIVE & FUNCTIONAL POLYMERS | 2004年 / 59卷 / 01期

关键词：

animated silica gel; polysuccinimide; enzyme support; porcine pancreatic lipase; immobilization;

D O I：

10.1016/j.reactfunctpolym.2003.12.013

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel enzyme support was prepared by the reaction between the amine attached on silica gel and polysuccinimide. The capacity of polysuccinimide on silica gel was 0.131 g/g of support. The excess polysuccinimide in the reaction solution to prepare support could be recycled. This shows potential practicability for bulk preparation of the support. Porcine pancreatic lipase (PPL) was immobilized on the support with a high activity recovery. The residual PPL in immobilization solution still kept high activity and was recyclable for the further usage of immobilization. Compared to free PPL the stability of immobilized PPL against thermal and pH of reaction medium was significantly improved. It was found that the immobilized PPL stored at 37 degreesC in phosphate buffer could keep its activity for a long time, and had higher activity than the one stored in 4 degreesC. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：93 / 98

页数：6

共 19 条

[1] Thermal stabilization of immobilized lipase B from Candida antarctica on different supports:: Effect of water activity on enzymatic activity in organic media [J].