Formation of Microchannels in Poly(ethylene glycol) Hydrogels by Selective Degradation of Patterned Microstructures

被引：20

作者：

Chiu, Yu-Chieh ^{[1
,2
]}

Larson, Jeffery C. ^{[2
]}

Perez-Luna, Victor H. ^{[1
]}

Brey, Eric A. ^{[2
,3
]}

机构：

[1] IIT, Dept Chem & Biol Engn, Chicago, IL 60616 USA

[2] IIT, Dept Biomed Engn, Chicago, IL 60616 USA

[3] Edward Hines Jr VA Hosp, Res Serv, Hines, IL 60141 USA

来源：

CHEMISTRY OF MATERIALS | 2009年 / 21卷 / 08期

基金：

美国国家科学基金会;

关键词：

CELL-ADHESIVE; STEM-CELLS; SURFACE; PROTEIN; PHOTOPOLYMERIZATION; NANOPARTICLES; FABRICATION; MONOLAYERS; PEPTIDES; NETWORKS;

D O I：

10.1021/cm803520j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A technique for constructing microchannels within three-dimensional hydrogels is described. The method depends on the use of two photopolymerizable macromers with different degradation kinetics. Poly(ethylene glycol)-diacrylate (PEG-DA) and poly(ethylene glycol)-co((L)-lactide) diacrylate (PEG-PLLA-DA) were synthesized and characterized by (HNMR)-H-1 and FTIR. PEG-PLLA (but not PEG-DA) gels degrade rapidly via hydrolysis. Gels were formed via bulk photopolymerization of the macromers by exposure to ultraviolet light in the presence of 2,2-dimethoxy-2-phenylacetophenone (DMPA) as an initiator. Patterns of PEG-PLLA-DA were generated within multilayer PEG hydrogels by noncontact photolithography. When the hydrogels were exposed to high pH conditions, the patterned PEG-PLLA-DA structures degraded rapidly, resulting in channels within the PEG-DA hydrogels. Single and multilayered channels were formed and their structure examined by phase contrast and confocal microscopy.

引用

页码：1677 / 1682

页数：6

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