Degradation markers of fibroin in silk through infrared spectroscopy

被引：111

作者：

Koperska, M. A. ^{[1
]}

Pawcenis, D. ^{[1
]}

Bagniuk, J. ^{[2
]}

Zaitz, M. M. ^{[1
]}

Missori, M. ^{[3
]}

Lojewski, T. ^{[1
]}

Lojewska, J. ^{[1
]}

机构：

[1] Jagiellonian Univ, Fac Chem, PL-30060 Krakow, Poland

[2] Jan Matejko Acad Fine Arts, PL-31157 Krakow, Poland

[3] CNR, Ist Sistemi Complessi, I-00015 Rome, Italy

来源：

POLYMER DEGRADATION AND STABILITY | 2014年 / 105卷

关键词：

Fibroin; ATR-FTIR spectroscopy; UV/Vis spectroscopy; XRD; SEC; Degradation estimators; FT-IR SPECTROSCOPY; NATURAL SILK; CROSS-LINKING; BOMBYX-MORI; RAMAN; CRYSTALLINITY; BEHAVIOR; NMR;

D O I：

10.1016/j.polymdegradstab.2014.04.008

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Infrared spectroscopy (FTIR) is a commonly available tool in laboratories dealing with both degradation of materials and conservation of art objects to evaluate their condition. In this paper FTIR was engaged to study degradation of fibroin included in silk samples (Bombyx mori) artificially aged at various conditions (oxygen, water vapor and volatile organic products) at temperature of 150 degrees C. The results collected by Attenuated Total Reflectance/Fourier Transform Infrared Spectroscopy (ATR/FTIR) were validated by UV/Vis, XRD and SEC analyses. Based on the results, the degradation estimators were verified and classified as: (1) crystallinity defined as ratio of absorbance at 1620 and at 1656 cm(-1), A(1620)/A(1656) [1], or at 1620 and 1699 cm(-1), A(1620)/A(1699) [2]; or ratio of areas under fitted curves of band at 1261 and 1230 cm(-1), P-1261/P-1230 [3], (2) oxidation - A(1620)/A(1514) and (3) depolymerization - P-1318/P-1442. Degradation gauges defined in such a way indicate that upon artificial aging water vapor and oxygen has the most profound impact on the progress of silk degradation. It also indicates that oxygen-lean conditions stabilize silk at elevated temperature. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：185 / 196

页数：12

共 49 条

[1] Tyrosinase-catalyzed grafting of sericin peptides onto chitosan and production of protein-polysaccharide bioconjugates [J].

Anghileri, Anna ;

Lantto, Raija ;

Kruus, Kristiina ;

Arosio, Cristina ;

Freddi, Giuliano .

JOURNAL OF BIOTECHNOLOGY, 2007, 127 (03) :508-519

[2]

[Anonymous], 2006, HDB FIBER CHEM

[3] Biodegradation of Bombyx mori silk fibroin fibers and films [J].

Arai, T ;

Freddi, G ;

Innocenti, R ;

Tsukada, M .

JOURNAL OF APPLIED POLYMER SCIENCE, 2004, 91 (04) :2383-2390

[4] Photochemical behaviour of natural silk - I. Kinetic investigation of photoyellowing [J].

Baltova, S ;

Vassileva, V ;

Valtcheva, E .

POLYMER DEGRADATION AND STABILITY, 1998, 60 (01) :53-60

[5]

Baltova S, 1998, POLYM DEGRAD STABIL, V61, P367

[6]

Becker B., 1989, ASC S SERIES, V410

[7] Solid state 13C NMR and FT-IR spectroscopy of the cocoon silk of two common spiders [J].

Bramanti, E ;

Catalano, D ;

Forte, C ;

Giovanneschi, M ;

Masetti, M ;

Veracini, CA .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2005, 62 (1-3) :105-111

[8] Resonance Raman examination of the electronic excited states of glycylglycine and other dipeptides: Observation of a carboxylate->amide charge transfer transition [J].

Chen, XG ;

Li, PS ;

Holtz, JSW ;

Chi, ZH ;

Pajcini, V ;

Asher, SA ;

Kelly, LA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (40) :9705-9715

[9] The photodegradation of some naturally occurring polymers [J].

Davidson, RS .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1996, 33 (01) :3-25

[10]

Fabina H, 2006, HDB VIBRATIONAL SPEC

← 1 2 3 4 5 →