The DnaK chaperone system facilitates 30S ribosomal subunit assembly

被引：74

作者：

Maki, JA ^{[1
]}

Schnobrich, DJ ^{[1
]}

Culver, GM ^{[1
]}

机构：

[1] Iowa State Univ Sci & Technol, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA

来源：

MOLECULAR CELL | 2002年 / 10卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(02)00562-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functional Escherichia coli 30S ribosomal subunits can be reconstituted in vitro. However, slow kinetics and sharp temperature dependence suggest additional assembly factors are present in vivo. Extract activation of in vitro assembly results in association of DnaK/hsp70 chaperone components with pre-30S particles. Purified DnaK, its cochaperones DnaJ and GrpE, and ATP can facilitate reconstitution of functional 30S subunits under otherwise nonpermissive conditions. A link has been observed between DnaK, 30S subunit components, and ribosome biogenesis in vivo as well as in vitro. These studies reveal a novel role for the DnaK/hsp70 chaperone system, in addition to its well-documented role in protein folding, and suggest that 30S subunit assembly can be facilitated.

引用

页码：129 / 138

页数：10

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