Ashton, REM and Twist, C. Number of directional changes alters the physiological, perceptual, and neuromuscular responses of netball players during intermittent shuttle running. J Strength Cond Res 29(10): 2731-2737, 2015This study investigated whether an increased number of changes in direction altered the metabolic, cardiovascular, perceptual, and neuromuscular responses to intermittent shuttle running (ISR). Using a randomized crossover design, 10 female netball players completed 30 minutes of ISR over a 10-m (ISR10) and 20-m (ISR20) linear course. Measures of expired air, heart rate (HR), rating of perceived exertion, blood lactate concentration ([BLa]), and peak torque of knee extensors and flexors were measured. Differences (%change +/- 90% CL) in V.o(2) (1.5 +/- 5.6%) was unclear between conditions, whereas HR was possibly higher (1.5 +/- 2.5%) and [BLa] very likely lower in ISR20 compared with ISR10 (-32.7 +/- 9.9%). Rating of perceived exertion was likely lower in the ISR20 compared with the ISR10 condition at 15 (-5.0 +/- 5.0%) and most likely lower at 30 minutes (-9.4 +/- 2.0%). Sprint times over 20 m were likely slower during ISR20 at mid (3.9 +/- 3.2%) but unclear after (2.1 +/- 5.4%). Changes in muscle function were not different between ISR10 and ISR20 conditions for knee extension (-0.2 +/- 0.9%) but were likely different for knee flexion (-5.7 +/- 4.9%). More directional changes during shuttle running increase the physiological and perceptual load on female athletes, which also cause a greater reduction in knee extensor torque. These findings have implications for the effective conditioning and injury prevention of female team sport athletes.