New recombinant of Tomato leaf curl New Delhi virus infecting melon in Indonesia

被引：9

作者：

Wilisiani, Fariha ^{[1
,2
]}

Mashiko, Takaaki ^{[1
]}

Wang, Wei-Qin ^{[3
]}

Suzuki, Tomohiro ^{[4
]}

Hartono, Sedyo ^{[5
]}

Neriya, Yutaro ^{[3
]}

Nishigawa, Hisashi ^{[1
,3
]}

Natsuaki, Tomohide ^{[1
,3
]}

机构：

[1] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Saiwai Cho, Fuchu, Tokyo 1838509, Japan

[2] Inst Agr STIPER, Fac Agr, Yogyakarta 55282, Indonesia

[3] Utsunomiya Univ, Sch Agr, Mine Machi 350, Utsunomiya, Tochigi 3218505, Japan

[4] Utsunomiya Univ, Ctr Biosci Res & Educ, Mine Machi 350, Utsunomiya, Tochigi 3218505, Japan

[5] Gadjah Mada Univ, Fac Agr, Bulaksumur 55281, Yogyakarta, Indonesia

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2019年 / 85卷 / 04期

关键词：

Melon; Begomovirus; ToLCNDV; Recombination; UNIVERSAL PRIMERS; DNA-BETA; BEGOMOVIRUS; DISEASE; AMPLIFICATION; CONSTRUCTION; COMPONENTS; ALIGNMENT;

D O I：

10.1007/s10327-019-00849-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The complete nucleotide sequence of Tomato leaf curl New Delhi virus detected from melon in Indonesia-[Indonesia:Yogya:Melon:2015] (ToLCNDV-[ID:YG:Mel:15]) was determined. ToLCNDV-[ID:YG:Mel:15] has bipartite DNAs, and both DNAs showed highest identity to ToLCNDV-[Indonesia:Java:Cucumber:2008] (ToLCNDV-[ID:JV:Cuc:08]). However in a phylogenetic analysis based on AV1, ToLCNDV-[ID:YG:Mel:15] clustered with Squash leaf curl China virus (SLCCNV) isolates. Recombination analyses revealed that ToLCNDV-[ID:YG:Mel:15] is a recombinant between ToLCNDV and SLCCNV. Thus, the virus is a new recombinant of ToLCNDV. The infectivity of the recombinant ToLCNDV-[ID:YG:Mel:15] isolate was confirmed, and the inoculation induced mosaic on melon, but not on tomato, the primary natural host of ToLCNDV.

引用

页码：306 / 310

页数：5

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