Silver Ion Unusually Stabilizes the Structure of a Parallel-Motif DNA Triplex

被引：114

作者：

Ihara, Toshihiro ^{[1
,3
]}

Ishii, Tatsuaki ^{[1
]}

Araki, Norie ^{[2
]}

Wilson, Anthony W. ^{[2
]}

Jyo, Akinori ^{[1
]}

机构：

[1] Kumamoto Univ, Grad Sch Sci & Technol, Kumamoto 8608555, Japan

[2] Kumamoto Univ, Grad Sch Med Sci, Kumamoto 8608556, Japan

[3] Japan Sci & Technol Agcy, PRESTO, Chiyoda Ku, Kawaguchi, Saitama 3320012, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 11期

关键词：

NUCLEIC-ACID; HELIX FORMATION; COMPLEXES; DUPLEXES; PAIRS;

D O I：

10.1021/ja809702n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple and easy method for stabilizing DNA triptexes using Ag(+) is reported. A silver ion displaces the N3 proton of cytosine in Hoogsteen base-pairing to form the base triplet CG.CAg(+). By the addition of an equimolar amount of Ag(+), the third-strand 15-mer sequence containing five cytosines was stabilized by similar to 30 degrees C in melting temperature at pH 7. The triplex structure was stable even under weakly basic conditions.

引用

页码：3826 / +

页数：3

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