Retention mechanism of peptides on a stationary phase embedded with a quaternary ammonium group: A liquid chromatography study

被引：43

作者：

Abbood, A. ^{[1
,2
]}

Smadja, C. ^{[1
]}

Herrenknecht, C. ^{[2
]}

Alahmad, Y. ^{[1
]}

Tchapla, A. ^{[3
]}

Taverna, M. ^{[1
]}

机构：

[1] Univ Paris Sud, Fac Pharm, JE 2494, F-92296 Chatenay Malabry, France

[2] Univ Paris Sud, CNRS, Lab Pharmacognosie, UMR 8076, F-92296 Chatenay Malabry, France

[3] Univ Paris 11, Grp Chim Analyt Paris Sud, LETIAM, IUT Orsay,EA 4041, F-91400 Orsay, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 15期

关键词：

Peptides; Mixed-mode chromatography; Positively charged embedded stationary phase; C18 stationary phase; PRINCIPAL COMPONENT ANALYSIS; ION-EXCHANGE CHROMATOGRAPHY; OVERLOADED BAND PROFILES; REVERSED-PHASE; MIXED-MODE; CAPILLARY ELECTROCHROMATOGRAPHY; COLUMN SELECTIVITY; PROTEOMIC ANALYSIS; MASS-SPECTROMETRY; PERFORMANCE;

D O I：

10.1016/j.chroma.2009.02.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the mechanisms involved in the retention of various peptides on a stationary phase embedded with a quaternary ammonium group (BS C23), by high-performance liquid chromatography. This was compared with peptide retention on a conventional reversed-phase C18 (RP C18) column under isocratic conditions, to understand better the various mechanisms involved. Chromatographic characterization of the two stationary phases with "model" compounds showed that BS C23 is less hydrophobic than RP C18 and induces electrostatic interaction (attraction or repulsion) with ionized compounds. If reversed-phase partitioning was the predominant retention phenomenon, for both stationary phases, the retention mechanisms in BS C23 provided different selectivity to that of RP C18. Electrostatic attraction or repulsion was clearly observed between peptides and the permanent positively charged group embedded in BS C23 depending on the pH. For most of the peptides, a weak anion-exchange mechanism was observed on the quaternary ammonium-embedded stationary phase if mobile phases at neutral pH and low ionic strengths were employed. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3244 / 3251

页数：8

共 43 条

[1] Retention pattern profiling of fungal metabolites on mixed-mode reversed-phase/weak anion exchange stationary phases in comparison to reversed-phase and weak anion exchange separation materials by liquid chromatography-electrospray ionisation-tandem mass spectrometry [J].

Apfelthaler, Elisabeth ;

Bicker, Wolfgang ;

Laemmerhofer, Michael ;

Sulyok, Michael ;

Krska, Rudolf ;

Lindner, Wolfgang ;

Schuhmacher, Rainer .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1191 (1-2) :171-181

[2] Capillary electrochromatography of peptides and proteins [J].

Bandilla, D ;

Skinner, CD .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1044 (1-2) :113-129

[3] NUCLEIC-ACID RESOLUTION BY MIXED-MODE CHROMATOGRAPHY [J].

BISCHOFF, R ;

MCLAUGHLIN, LW .

JOURNAL OF CHROMATOGRAPHY, 1984, 296 (JUL) :329-337

[4] THE S INDEX IN THE RETENTION EQUATION IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

CHEN, N ;

ZHANG, YK ;

LU, PC .

JOURNAL OF CHROMATOGRAPHY, 1992, 603 (1-2) :35-42

[5] Comparison of retention on traditional alkyl, polar endcapped, and polar embedded group stationary phases [J].

Coym, Jason W. .

JOURNAL OF SEPARATION SCIENCE, 2008, 31 (10) :1712-1718

[6] A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography [J].

Davies, Nicola H. ;

Euerby, Melvin R. ;

McCalley, David V. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1138 (1-2) :65-72

[7] Reversed-phase and weak anion-exchange mixed-mode silica-based monolithic column for capillary electrochromatography [J].

Ding, Guosheng ;

Da, Zhenliang ;

Yuan, Ruijuan ;

Bao, James J. .

ELECTROPHORESIS, 2006, 27 (17) :3363-3372

[8]

Engelhardt H, 1997, LC GC-MAG SEP SCI, V15, P856

[9] Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns using principal component analysis [J].

Euerby, M ;

Petersson, P .

JOURNAL OF CHROMATOGRAPHY A, 2003, 994 (1-2) :13-36

[10] Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns containing polar embedded groups/amino endcappings using principal component analysis [J].

Euerby, MR ;

Petersson, P .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1088 (1-2) :1-15

← 1 2 3 4 5 →