High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis

被引：21

作者：

Cazal, Cristiane de Melo ^{[1
]}

Batalhao, Jaqueline Raquel ^{[1
]}

Domingues, Vanessa de Cassia ^{[1
]}

Bueno, Odair Correa ^{[1
]}

Rodrigues Filho, Edson ^{[1
]}

Forim, Moacir R. ^{[1
]}

Fernandes da Silva, Maria Fatima G. ^{[1
]}

Vieira, Paulo Cezar ^{[1
]}

Fernandes, Joao Batista ^{[1
]}

机构：

[1] Univ Fed Sao Carlos, Dept Quim, Lab Prod Nat, BR-13565905 Sao Carlos, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 19期

基金：

巴西圣保罗研究基金会;

关键词：

High-speed counter-current chromatography; Ricinine; Leaf-cutting ant; Ricinus communis; EUPHORBIACEAE;

D O I：

10.1016/j.chroma.2009.02.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The alkaloid ricinine, an insecticide for leaf-cutting ant (Atta sexdens rubropilosa), was obtained from Ricinus communis. A two-phase solvent system composed of CH2Cl2/EtOH/H2O (93:35:72, v/v/v) was used for high-speed counter-current chromatographic (HSCCC) isolation of ricinine in high yield and with over 96% purity, as determined by liquid and gas chromatography-mass spectrometry (LC-MS and CC-MS). Identification of ricinine was performed by comparison of H-1 NMR, C-13 NMR and LC-MS/MS data. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：4290 / 4294

页数：5

共 17 条

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