Human Telomerase RNA Processing and Quality Control

被引：119

作者：

Tseng, Chi-Kang ^{[1
,2
]}

Wang, Hui-Fang ^{[1
,2
]}

Burns, Allison M. ^{[2
]}

Schroeder, Morgan R. ^{[2
]}

Gaspari, Martina ^{[2
]}

Baumann, Peter ^{[1
,2
,3
]}

机构：

[1] Howard Hughes Med Inst, Kansas City, MO 64110 USA

[2] Stowers Inst Med Res, Kansas City, MO 64110 USA

[3] Univ Kansas, Med Ctr, Dept Mol & Integrat Physiol, Kansas City, KS 66160 USA

来源：

CELL REPORTS | 2015年 / 13卷 / 10期

关键词：

H/ACA SMALL NUCLEOLAR; CAP-BINDING COMPLEX; NUCLEAR EXOSOME; 3' END; TRANSCRIPTION TERMINATION; APLASTIC-ANEMIA; EXON LIGATION; MUTATIONS; BIOGENESIS; YEAST;

D O I：

10.1016/j.celrep.2015.10.075

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The non-coding RNA subunit of telomerase provides the template for telomerase activity. In diverse fungi, 30 end processing of telomerase RNA involves a single cleavage by the spliceosome. Here, we examine how human telomerase RNA (hTR) primary transcripts are processed into the mature form of precisely 451 nt. We find that the splicing inhibitor isoginkgetin mimics the effects of RNA exosome inhibition and causes accumulation of long hTR transcripts. Depletion of exosome components and accessory factors reveals functions for the cap binding complex (CBC) and the nuclear exosome targeting (NEXT) complex in hTR turnover. Whereas longer transcripts are predominantly degraded, shorter precursor RNAs are oligo-adenylated by TRF4-2 and either processed by poly(A)-specific ribonuclease (PARN) or degraded by the exosome. Our results reveal that hTR biogenesis involves a kinetic competition between RNA processing and degradation and suggest treatment options for telomerase insufficiency disorders.

引用

页码：2232 / 2243

页数：12

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