Epidermal growth factor upregulates serotonin transporter and its association with visceral hypersensitivity in irritable bowel syndrome

被引：19
作者：
Cui, Xiu-Fang [1 ]
Zhou, Wei-Mei [1 ]
Yang, Yan [1 ]
Zhou, Jun [1 ]
Li, Xue-Liang [1 ]
Lin, Lin [1 ]
Zhang, Hong-Jie [1 ]
机构：
[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Nanjing 210029, Jiangsu, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2014年 / 20卷 / 37期
基金：
中国国家自然科学基金;
关键词：
Epidermal growth factor; Visceral hypersensitivity; Rat models; Serotonin transporter; Rat small intestinal epithelial cells; intestinal epithelial cells; Irritable bowel syndrome; INTESTINAL EPITHELIAL-CELLS; COLON IRRITATION; MESSENGER-RNA; EXPRESSION; RECEPTOR; MUCOSA; COLITIS; DISEASE; RATS; PROTECTION;
D O I：
10.3748/wjg.v20.i37.13521
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To investigate the role of epidermal growth factor (EGF) in visceral hypersensitivity and its effect on the serotonin transporter (SERT). METHODS: A rat model for visceral hypersensitivity was established by intra-colonic infusion of 0.5% acetic acid in 10-d-old Sprague-Dawley rats. The visceral sensitivity was assessed by observing the abdominal withdrawal reflex and recording electromyographic activity of the external oblique muscle in response to colorectal distension. An enzyme-linked immunosorbent assay was used to measure the EGF levels in plasma and colonic tissues. SERT mRNA expression was detected by real-time PCR while protein level was determined by Western blot. The correlation between EGF and SERT levels in colon tissues was analyzed by Pearson's correlation analysis. SERT function was examined by tritiated serotonin (5-HT) uptake experiments. Rat intestinal epithelial cells (IEC-6) were used to examine the EGF regulatory effect on SERT expression and function via the EGF receptor (EGFR). RESULTS: EGF levels were significantly lower in the rats with visceral hypersensitivity as measured in plasma (2.639 +/- 0.107 ng/mL vs 4.066 +/- 0.573 ng/mL, P < 0.01) and in colonic tissue (3.244 +/- 0.135 ng/100 mg vs 3.582 +/- 0.197 ng/100 mg colon tissue, P < 0.01) compared with controls. Moreover, the EGF levels were positively correlated with SERT levels (r = 0.820, P < 0.01). EGF displayed dose-and time-dependent increased SERT gene expressions in IEC-6 cells. An EGFR kinase inhibitor inhibited the effect of EGF on SERT gene upregulation. SERT activity was enhanced following treatment with EGF (592.908 +/- 31.515 fmol/min per milligram vs 316.789 +/- 85.652 fmol/min per milligram protein, P < 0.05) and blocked by the EGFR kinase inhibitor in IEC-6 cells (590.274 +/- 25.954 fmol/min per milligram vs 367.834 +/- 1 20.307 fmol/min per milligram protein, P < 0.05). CONCLUSION: A decrease in EGF levels may contribute to the formation of visceral hypersensitivity through downregulation of SERT-mediated 5-HT uptake into enterocytes. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.
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页码：13521 / 13529
页数：9
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