Comparative transcriptome analysis of male and female flowers in Spinacia oleracea L

被引:22
|
作者
Li, Ning [1 ]
Meng, Ziwei [1 ]
Tao, Minjie [1 ]
Wang, Yueyuan [1 ]
Zhang, Yulan [1 ]
Li, Shufen [1 ]
Gao, Wujun [1 ]
Deng, Chuanliang [1 ]
机构
[1] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
Spinach; Sex determination and differentiation; Gibberellin; Auxin; Sugar; Full-length transcriptome; SUCROSE-SPECIFIC INDUCTION; GENOME-WIDE ANALYSIS; SEX DETERMINATION; GENE-EXPRESSION; MESSENGER-RNA; Y-CHROMOSOME; ANTHOCYANIN BIOSYNTHESIS; MALE-STERILITY; MALE-FERTILITY; ARABIDOPSIS;
D O I
10.1186/s12864-020-07277-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background Dioecious spinach (Spinacia oleracea L.), a commercial and nutritional vegetable crop, serves as a model for studying the mechanisms of sex determination and differentiation in plants. However, this mechanism is still unclear. Herein, based on PacBio Iso-seq and Illumina RNA-seq data, comparative transcriptome analysis of male and female flowers were performed to explore the sex differentiation mechanism in spinach. Results Compared with published genome of spinach, 10,800 transcripts were newly annotated; alternative splicing, alternative polyadenylation and lncRNA were analyzed for the first time, increasing the diversity of spinach transcriptome. A total of 2965 differentially expressed genes were identified between female and male flowers at three early development stages. The differential expression of RNA splicing-related genes, polyadenylation-related genes and lncRNAs suggested the involvement of alternative splicing, alternative polyadenylation and lncRNA in sex differentiation. Moreover, 1946 male-biased genes and 961 female-biased genes were found and several candidate genes related to gender development were identified, providing new clues to reveal the mechanism of sex differentiation. In addition, weighted gene co-expression network analysis showed that auxin and gibberellin were the common crucial factors in regulating female or male flower development; however, the closely co-expressed genes of these two factors were different between male and female flower, which may result in spinach sex differentiation. Conclusions In this study, 10,800 transcripts were newly annotated, and the alternative splicing, alternative polyadenylation and long-noncoding RNA were comprehensively analyzed for the first time in spinach, providing valuable information for functional genome study. Moreover, candidate genes related to gender development were identified, shedding new insight on studying the mechanism of sex determination and differentiation in plant.
引用
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页数:19
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