Sip5 interacts with both the Reg1/Glc7 protein phosphatase and the Snf1 protein kinase of Saccharomyces cerevisiae

被引:0
作者
Sanz, P
Ludin, K
Carlson, M
机构
[1] Columbia Univ, Dept Genet, New York, NY 10032 USA
[2] Columbia Univ, Dept Dev, New York, NY 10032 USA
[3] Columbia Univ, Dept Microbiol, New York, NY 10032 USA
[4] CSIC, Inst Biomed Valencia, Valencia, Spain
关键词
D O I
暂无
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The Snf1 protein kinase is an essential component of thr glucose starvation signalling pathway in Saccharomyces cerevisiae. We have used the two-hybrid system to identify a new protein, Sip5, that interacts with the Snf1 kinase complex in response to glucose limitation. Coimmunoprecipitation studies confirmed the association of Sip5 and Snf1 in cell extracts. We found that Sip5 also interacts strongly with Reg1, the regulatory subunit of the Reg1/Glc7 protein phosphatase 1 complex, in both two-hybrid and coimmunoprecipitation assays. Previous work showed that Reg1/Glc7 interacts with the Snf1 kinase under glucose-limiting conditions and negatively regulates its activity. Sip5 is the first protein that has been shown to interact with both Snf1 and Reg1/Glc7. Genetic analysis showed that the two-hybrid intel action between Reg1 and Snf1 is reduced threefold in a sip5 Delta mutant. These findings suggest that Sip5 facilitates the interaction between the Reg1/Glc7 phosphatase and the Snf1 kinase.
引用
收藏
页码:99 / 107
页数:9
相关论文
共 32 条
  • [1] ALANI E, 1987, GENETICS, V117, P5
  • [2] Reg1p targets protein phosphatase 1 to dephosphorylate hexokinase II in Saccharomyces cerevisiae:: characterizing the effects of a phosphatase subunit on the yeast proteome
    Alms, GR
    Sanz, P
    Carlson, M
    Haystead, TAJ
    [J]. EMBO JOURNAL, 1999, 18 (15) : 4157 - 4168
  • [3] THE YDP PLASMIDS - A UNIFORM SET OF VECTORS BEARING VERSATILE GENE DISRUPTION CASSETTES FOR SACCHAROMYCES-CEREVISIAE
    BERBEN, G
    DUMONT, J
    GILLIQUET, V
    BOLLE, PA
    HILGER, F
    [J]. YEAST, 1991, 7 (05) : 475 - 477
  • [4] ANALYSIS OF RIM11, A YEAST PROTEIN-KINASE THAT PHOSPHORYLATES THE MEIOTIC ACTIVATOR IME1
    BOWDISH, KS
    YUAN, HE
    MITCHELL, AP
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (12) : 7909 - 7919
  • [5] MUTATIONAL ANALYSIS OF THE SACCHAROMYCES-CEREVISIAE SNF1 PROTEIN-KINASE AND EVIDENCE FOR FUNCTIONAL INTERACTION WITH THE SNF4 PROTEIN
    CELENZA, JL
    CARLSON, M
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) : 5034 - 5044
  • [6] A YEAST GENE THAT IS ESSENTIAL FOR RELEASE FROM GLUCOSE REPRESSION ENCODES A PROTEIN-KINASE
    CELENZA, JL
    CARLSON, M
    [J]. SCIENCE, 1986, 233 (4769) : 1175 - 1180
  • [7] MULTIFUNCTIONAL YEAST HIGH-COPY-NUMBER SHUTTLE VECTORS
    CHRISTIANSON, TW
    SIKORSKI, RS
    DANTE, M
    SHERO, JH
    HIETER, P
    [J]. GENE, 1992, 110 (01) : 119 - 122
  • [8] SIMILAR SUBSTRATE RECOGNITION MOTIFS FOR MAMMALIAN AMP-ACTIVATED PROTEIN-KINASE, HIGHER-PLANT HMG-COA REDUCTASE KINASE-A, YEAST SNF1, AND MAMMALIAN CALMODULIN-DEPENDENT PROTEIN-KINASE-I
    DALE, S
    WILSON, WA
    EDELMAN, AM
    HARDIE, DG
    [J]. FEBS LETTERS, 1995, 361 (2-3) : 191 - 195
  • [9] THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT
    DURFEE, T
    BECHERER, K
    CHEN, PL
    YEH, SH
    YANG, YZ
    KILBURN, AE
    LEE, WH
    ELLEDGE, SJ
    [J]. GENES & DEVELOPMENT, 1993, 7 (04) : 555 - 569
  • [10] LAMBDA-YES - A MULTIFUNCTIONAL CDNA EXPRESSION VECTOR FOR THE ISOLATION OF GENES BY COMPLEMENTATION OF YEAST AND ESCHERICHIA-COLI MUTATIONS
    ELLEDGE, SJ
    MULLIGAN, JT
    RAMER, SW
    SPOTTSWOOD, M
    DAVIS, RW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) : 1731 - 1735