Engineering fatty acid synthases for directed polyketide production

被引：52
作者：
Gajewski, Jan [1 ]
Buelens, Floris [2 ]
Serdjukow, Sascha [3 ]
Janssen, Melanie [1 ]
Cortina, Nina [1 ]
Grubmueller, Helmut [2 ]
Grininger, Martin [1 ,3 ]
机构：
[1] Goethe Univ Frankfurt, Inst Organ Chem & Chem Biol, Buchmann Inst Mol Life Sci, Cluster Excellence Macromol Complexes, Frankfurt, Germany
[2] Max Planck Inst Biophys Chem, Dept Theoret & Computat Biophys, Gottingen, Germany
[3] Max Planck Inst Biochem, Project Grp Biol Chem, Martinsried, Germany
来源：
NATURE CHEMICAL BIOLOGY | 2017年 / 13卷 / 04期
关键词：
ACYL CARRIER PROTEIN; STRUCTURAL BASIS; YEAST; SPECIFICITY; SUBSTRATE; BIOSYNTHESIS; INHIBITION; SYNTHETASE; LACTONE; COA;
D O I：
10.1038/nchembio.2314
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.
引用
收藏
页码：363 / +
页数：7
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