MiR-26b-3p regulates osteoblast differentiation via targeting estrogen receptor α

被引:22
作者
Lin, Yu [1 ]
Xiao, Lili [1 ]
Zhang, Yiyuan [1 ]
Li, Ping [1 ]
Wu, Yinsheng [2 ]
Lin, Yanping [2 ]
机构
[1] Xiamen Univ, Dept Orthopaed, Hosp Fuzhou 2, Fuzhou 350007, Fujian, Peoples R China
[2] Fujian Tradit Chinese Med Univ Integrated Tradit, Inst Osteopathy, Western Med Res Inst, Fuzhou 350102, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
miR-26b-3p; Osteoblast differentiation; Estrogen receptor alpha; Bone regeneration; GENE-EXPRESSION; CELL-PROLIFERATION; OSTEOCALCIN GENE; TRANSCRIPTION; WNT; ACTIVATION; REPRESSION; INDUCTION; BMP-2;
D O I
10.1016/j.ygeno.2018.07.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Understanding of the molecular mechanisms of miRNAs involved in osteoblast differentiation is important for the treatment of bone-related diseases. Methods: MC3T3-E1 cells were induced to osteogenic differentiation by culturing with bone morphogenetic protein 2 (BMP2). After transfected with miR-26b-3p mimics or inhibitors, the osteogenic differentiation of MC3T3-E1 cells was detected by ALP and ARS staining. Cell viability was analyzed by MTT. The expressions of miR-26b-3p and osteogenic related markers and signaling were examined by qPCR and western blot. Direct binding of miR-26b-3p and ER-alpha were determined by dual luciferase assay. Results: miR-26b-3p was significantly down-regulated during osteoblast differentiation. Overexpression of miR-26b-3p inhibited osteoblast differentiation, while inhibition of miR-26b-3p enhanced osteoblast differentiation. Further studies demonstrated miR-26b-3p inhibited the expression of estrogen receptor a (ER-alpha) by directly targeting to the CDS region of ER-alpha mRNA. Overexpression of ER-alpha rescued the suppression effects of miR-26b-3p on osteoblast differentiation, while knockdown of ER-alpha reversed the upregulation of osteoblast differentiation induced by knockdown of miR-26b-3p. Conclusion: Our study demonstrates that miR-26b-3p suppresses osteoblast differentiation of MC3T3-E1 cells via directly targeting ER-alpha.
引用
收藏
页码:1089 / 1096
页数:8
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