Graphene nanoparticles induces apoptosis in MCF-7 cells through mitochondrial damage and NF-KB pathway

被引:42
|
作者
Alsaedi, Iman I. J. [1 ]
Taqi, Zainab J. [2 ]
Hussien, Adi M. Abdul [3 ]
Sulaiman, Ghassan M. [1 ]
Jabir, Majid S. [1 ]
机构
[1] Univ Technol Baghdad, Appl Sci Dept, Biotechnol Div, Baghdad, Iraq
[2] Univ Technol Baghdad, Appl Sci Dept, Appl Chem Div, Baghdad, Iraq
[3] Univ Technol Baghdad, Appl Sci Dept, Appl Phys Div, Baghdad, Iraq
来源
MATERIALS RESEARCH EXPRESS | 2019年 / 6卷 / 09期
关键词
rGO; MCF-7; cells; apoptosis; mitochondria; NF-kappa B; Bax/Bcl-2; IN-VITRO EVALUATION; ANTICANCER ACTIVITY; OXIDE; AUTOPHAGY; CARBON;
D O I
10.1088/2053-1591/ab33af
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
In the present study, the synthesis of reduced graphene oxide (rGO) was performed via 20 kHz frequency ultrasonic solution processing technique. Synthesis of rGo was confirmed by various techniques including color changes, UV-vis spectra, x-ray analysis, infrared spectrophotometry, scanning electron microscopy, and dynamic light-scattering. The cytotoxicity of rGO was examined against human breast cancer MCF-7 cells by measuring different parameters including MTT, suppression of NF-kappa B translocation, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, acridine orange-ethidium bromide staining, and single cell gel electrophoresis. Also, the gene expressions of Bax and Bcl-2 proteins were measured using quantitative PCR analysis as well as florescence microscopy and indicated that rGO induces cell death using apoptosis as an exclusive mechanism. Our results shows for the first time that the rGO inhibited the proliferation of MCF-7 cells, leading to programmed cell death through activation of the mitochondrial-mediated signaling pathway with the involvement of the NF-kB signalling pathway. Taken together the present data suggest that rGO can be possibly employed in the treatment of breast cancer with the function of a potent synergistic agent added to anticancer therapy protocols.
引用
收藏
页数:13
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