In vitro oxidation of LDL by ozone

被引：2

作者：

Hoerl, Gerd ^{[1
]}

Ledinski, Gerhard ^{[1
]}

Kager, Gerd ^{[1
]}

Hallstroem, Seth ^{[1
]}

Tafeit, Erwin ^{[1
]}

Koestenberger, Martin ^{[2
]}

Juergens, Guenther ^{[1
]}

Cvirn, Gerhard ^{[1
]}

机构：

[1] Med Univ Graz, Inst Physiol Chem, A-8010 Graz, Austria

[2] Med Univ Graz, Dept Pediat, A-8010 Graz, Austria

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2014年 / 183卷

关键词：

Atherosclerosis; Lipid hydroperoxides; Low-density lipoprotein; Oxidation-specific immune epitopes; Ozone; LOW-DENSITY-LIPOPROTEIN; GENERATION; PATHWAY; ACIDS; VIVO;

D O I：

10.1016/j.chemphyslip.2014.05.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies suggest that ozone is present in atherosclerotic lesions. Since these lesions are characterized by a dramatic accumulation of low-density lipoprotein (LDL), we aimed to investigate whether ozone is capable of oxidizing LDL, thereby rendering this lipoprotein atherogenic. Lipid hydroperoxide (LPO) concentrations and thiobarbituric acid reactive substances (TBARS) were measured to assess the oxidative status of the lipid part of LDL. Relative electrophoretic mobility (REM) and oxidation-specific immune epitopes were measured to assess the oxidative status of the protein part (apoB) of the LDL particle. Ozone turned out to be a potent oxidant of LDL. LPO concentrations, TBARS, REM, and oxidation-specific immune epitopes significantly increased upon ozonization. Our results suggest that ozonization of LDL may be a novel pathway which supports atherogenesis. Ozone is capable of oxidizing the lipid part of LDL, followed by immediate oxidation of the protein part of LDL, rendering the lipoprotein atherogenic. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：18 / 21

页数：4

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