Effect of environmental tobacco smoke on intracellular free calcium of boar sperm incubated in seminal plasma

被引：1

作者：

Zhou, RQ

Zhou, YQ

Li, SB

Haug, A

机构：

[1] Michigan State Univ, Pesticide Res Ctr, E Lansing, MI 48824 USA

[2] Michigan State Univ, Dept Microbiol, E Lansing, MI 48824 USA

[3] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110015, Peoples R China

[4] Liaoning Univ, Dept Biol, Shenyang 110036, Peoples R China

来源：

TOXICOLOGY LETTERS | 2000年 / 114卷 / 1-3期

关键词：

boar sperm; intracellular Ca2+; environmental tobacco smoke;

D O I：

10.1016/S0378-4274(99)00291-X

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The extractable-respirable particulate of environmental tobacco smoke (ERP-ETS) was collected and tested for its effect on the intracellular free Ca2+ concentration, [Ca2+](i), in boar sperm incubated in seminal plasma in vitro. The intracellular free Ca2+ level is reportedly closely related to in sperm capacitation and acrosome reaction, which must take place to confer upon sperm the full fertilizing potential. In the absence of ERP-ETS [Ca2+](i) in the boar sperm increased dramatically from initially 75 nM to about 248 nM after a 5-h incubation in seminal plasma. However, such an increase was virtually inhibited by ERP-ETS at a concentration of 10 mu g/ml, without any significant reduction of the sperm motility and viability. We conclude that application of ERP-ETS negatively affected the [Ca2+](i) of boar sperm in vitro. Published by Elsevier Science Ireland Ltd.

引用

页码：135 / 141

页数：7

共 23 条

[1] DEPOLARIZATION OF HUMAN NEURO-BLASTOMA CELLS AS A RESULT OF MUSCARINIC RECEPTOR-INDUCED RISE IN CYTOSOLIC CA-2+ [J].