Voltage-gated sodium channel expressed in cultured human smooth muscle cells: involvement of SCN9A

被引：44

作者：

Jo, T ^{[1
]}

Nagata, T ^{[1
]}

Iida, H ^{[1
]}

Imuta, H ^{[1
]}

Iwasawa, K ^{[1
]}

Ma, J ^{[1
]}

Hara, K ^{[1
]}

Omata, M ^{[1
]}

Nagai, R ^{[1
]}

Takizawa, H ^{[1
]}

Nagase, T ^{[1
]}

Nakajima, T ^{[1
]}

机构：

[1] Univ Tokyo, Dept Cardiovasc Resp Med & Gastroenterol, Bunkyo Ku, Tokyo 1138655, Japan

来源：

FEBS LETTERS | 2004年 / 567卷 / 2-3期

关键词：

voltage-gated sodium channel; cultured human; smooth muscle cell; SCN9A; Na(v)1.7; RT-PCR; tetrodotoxin;

D O I：

10.1016/j.febslet.2004.04.092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Voltage-gated Na+ channel (I-Na) is expressed under culture conditions in human smooth muscle cells (hSMCs) such as coronarv myocytes. The aim of this study is to clarify the physiological, pharmacological and molecular characteristics of I-Na expressed in cultured hSMCs obtained from bronchus, main pulmonary and coronarv artery. I-Na, was recorded in these hSMCs and inhibited by tetrodotoxin (TTX) with an IC50 value of approximately 10 nM. Reverse transcriptase/polymerase chain reaction (RT-PCR) analysis of mRNA showed the prominent expression of transcripts for SCN9A, which was consistent with the results of real-time quantitative RT-PCR. These results provide novel evidence that TTX-sensitive Na+ channel expressed in cultured hSMCs is mainly composed of Na(v)1.7. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：339 / 343

页数：5

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