Detection of Babesia bigemina DNA in ticks by DNA hybridization using a nonradioactive probe generated by arbitrary PCR

被引：12

作者：

Ravindran, Reghu ^{[1
]}

Rao, Jammi R. ^{[1
]}

Mishra, Ashok K. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Parasitol, Izatnagar 243122, Uttar Pradesh, India

来源：

VETERINARY PARASITOLOGY | 2006年 / 141卷 / 1-2期

关键词：

DNA probe; Babesia bigemina; ticks; RAPD-PCR;

D O I：

10.1016/j.vetpar.2006.04.033

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The Dig-labeled probe specific to Babesia bigemina generated from monomorphic RAPD fragment of similar to 873 by size amplified by a 10 mer CGGTGGCGAA, detected up to 100 ng of template DNA. This nonradioactive probe also detected B. bigemina in preparations of larval tick DNA from two of the five samples on dot-blot hybridization. 2006 Elsevier B.V. All rights reserved.

引用

页码：181 / 185

页数：5

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