miR-338-3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β-catenin signaling pathways

The aim of the present study was to explore the underlying mechanism of microRNA-338-3p (miR-338-3p) in lung cancer cell (A549) invasion and proliferation. A microarray assay showed that miR-338-3p upregulated 216 and downregulated 147 genes in A549 cells, and the differentially expressed genes were enriched for several signaling pathways, including AKT and beta-catenin signaling pathways. Western blotting results showed that phosphorylated (p)-AKT at Ser473 and at Thr308 and p-beta-catenin at Ser552 were downregulated. Inhibiting AKT signaling pathway decreased beta-catenin signaling pathway. Overexpression of beta-catenin promoted the invasion of A549 cells. In addition, miR-338-3p showed inhibitory effect on the primary tumor developed from Kras(G12D) mice. Together, the data of the present study support that miR-338-3p inhibits lung cancer cell invasion and proliferation by downregulating AKT/beta-catenin signaling pathway. The present findings supported the potential use of miR-338-3p in the treatment of lung cancer.