Development of Microsatellite Markers in Sea Perch, Lateolabrax Japonicus, from Codominant Amplified Fragment Length Polymorphism Bands

A fast and cost-effective protocol to develop candidate microsatellite markers from sea perch, Lateolabrax japonicus, was described here. Ten suites of codominant bands that contained seven microsatellites were discovered in this marine fish, in which no microsatellite development was reported previously. All the seven microsatellites were found to be polymorphic among tested 20 individuals of sea perch. Five of the above seven loci conformed to Hardy-Weinberg equilibrium (HWE) as determined by using the Markov-Chain method implemented, the other two loci significantly deviated from HWE, both of them showed a large heterozygote excess (H-E). Out of 21 possible pair-wise comparisons among the seven loci applied to sea perch, none showed significant linkage disequilibrium (LD) (P > 0.008). Five additional fish species assessed for cross-species amplification revealed that some of the loci appear to be applicable in close-related species.