Point-of-Care Fluorescence Immunoassay for Cardiac Panel Biomarkers

被引:14
作者
Kim, Tae Kyum [1 ,2 ]
Oh, Sang Wook [3 ]
Hong, Soon Cheol [2 ]
Mok, Young Joon [2 ]
Choi, Eui Yul [1 ,2 ]
机构
[1] Hallym Univ, Dept Biomed Sci, Chunchon 200702, South Korea
[2] BodiTech Med Inc, Chunchon, South Korea
[3] Chonbuk Natl Univ, Inst Fus Sci, Dept Biol Educ, Jeonju, South Korea
关键词
AMI; CK-MB; cTnI; fluorescence immunochromatographic assay; myoglobin; POCT; ACUTE MYOCARDIAL-INFARCTION; TROPONIN-I LEVELS; EMERGENCY-DEPARTMENT; MULTIPLE BIOMARKERS; CK-MB; ASSAY; PREDICTION; MYOGLOBIN; DIAGNOSIS; MARKERS;
D O I
10.1002/jcla.21704
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Myoglobin, creatine kinase-MB isoenzyme (CK-MB), and cardiac troponin I (cTnI) are cardiac biomarkers that are widely used to assist in the early and late diagnoses of acute myocardial infarction (AMI). Here, we present a clinically applicable fluorescence (FL) immunoassay for cardiac biomarkers. Methods: Whole blood was mixed with FL-labeled detector Ab (antibody) and then loaded onto a capture Abimmobilized strip in a test cartridge. The FL intensities at test and control line on the strip were obtained and converted in a laser FL scanner to determine the concentration of biomarker. The analytical performance of immunoassay system was evaluated by linearity and imprecision tests. The comparability of the FL immunoassay method was examined with a reference method. Results: FL intensities and the levels of myoglobin, CK-MB, and cTnI displayed good linearity and high correlations (r = 0.999, 0.998, and 0.989, respectively). The coefficient of variations (CVs) of imprecision for all cardiac biomarkers were less than 8% in both intra-and interassays. When the results from the developed method and bioMerieux VIDAS assay were analyzed by Bland-Altman plot and Passing-Bablok plot, the two assay methods were in good agreement. Conclusion: The FL immunoassay system can provide a platform for point-of-care testing (POCT), and it is an easy, fast, and reliable method for the quantification of cardiac biomarkers. C (C) 2014 Wiley Periodicals, Inc.
引用
收藏
页码:419 / 427
页数:9
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