Rapid typing of Bordetella pertussis pertussis toxin gene variants by LightCycler real-time PCR and fluorescence resonance energy transfer hybridization probe melting curve analysis

被引：12
作者：
Mäkinen, J
Mertsola, J
Viljanen, MK
Arvilommi, H
He, QS
机构：
[1] Univ Turku, Natl Publ Hlth Inst, Pertussis Reference Lab, Dept Turku, FIN-20520 Turku, Finland
[2] Univ Turku, Turku Grad Sch Biomed Sci, FIN-20520 Turku, Finland
[3] Univ Turku, Dept Pediat, FIN-20520 Turku, Finland
[4] Univ Turku, Dept Med Microbiol, FIN-20520 Turku, Finland
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 06期
关键词：
D O I：
10.1128/JCM.40.6.2213-2216.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A LightCycler real-time PCR hybridization probe assay was developed for rapid typing of gene variants of the Bordetella pertussis virulence factor pertussis toxin. The assay correctly identified the ptxS1 alleles of all strains tested, comprising 57 Finnish clinical isolates and 2 vaccine strains. The method is simple, reliable, and suitable for large-scale screening of B. pertussis strains.
引用
收藏
页码：2213 / 2216
页数：4
相关论文
共 25 条
[1]  
Andrews R, 1997, Commun Dis Intell, V21, P145
[2]   ANTIGENIC RELATIONSHIP BETWEEN SEROTYPE-SPECIFIC AGGLUTINOGEN AND FIMBRIAE OF BORDETELLA-PERTUSSIS [J].
ASHWORTH, LAE ;
IRONS, LI ;
DOWSETT, AB .
INFECTION AND IMMUNITY, 1982, 37 (03) :1278-1281
[3]   Epidemiology of pertussis in French hospitals in 1993 and 1994: thirty years after a routine use of vaccination [J].
Baron, S ;
Njamkepo, E ;
Grimprel, E ;
Begue, P ;
Desenclos, JC ;
Drucker, J ;
Guiso, N .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1998, 17 (05) :412-418
[4]   RETURN OF EPIDEMIC PERTUSSIS IN THE UNITED-STATES [J].
BASS, JW ;
WITTLER, RR .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1994, 13 (05) :343-345
[5]   Polymorphism in Bordetella pertussis pertactin and pertussis toxin virulence factors in the United States, 1935-1999 [J].
Cassiday, P ;
Sanden, G ;
Heuvelman, K ;
Mooi, F ;
Bisgard, KM ;
Popovic, T .
JOURNAL OF INFECTIOUS DISEASES, 2000, 182 (05) :1402-1408
[6]  
*CDCP, 1998, PREV EM INF DIS STRA
[7]  
CHARLES I G, 1988, Tokai Journal of Experimental and Clinical Medicine, V13, P227
[8]   MOLECULAR-CLONING AND CHARACTERIZATION OF PROTECTIVE OUTER-MEMBRANE PROTEIN-P.69 FROM BORDETELLA-PERTUSSIS [J].
CHARLES, IG ;
DOUGAN, G ;
PICKARD, D ;
CHATFIELD, S ;
SMITH, M ;
NOVOTNY, P ;
MORRISSEY, P ;
FAIRWEATHER, NF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3554-3558
[9]   Pertussis in the Netherlands: An outbreak despite high levels of immunization with whole-cell vaccine [J].
deMelker, HE ;
ConynvanSpaendonck, MAE ;
Rumke, H ;
vanWijngaarden, JK ;
Mooi, FR ;
Schellekens, JFP .
EMERGING INFECTIOUS DISEASES, 1997, 3 (02) :175-178
[10]   Genotypic variation in the Bordetella pertussis virulence factors pertactin and pertussis toxin in historical and recent clinical isolates in the United Kingdom [J].
Fry, NK ;
Neal, S ;
Harrison, TG ;
Miller, E ;
Matthews, R ;
George, RC .
INFECTION AND IMMUNITY, 2001, 69 (09) :5520-5528
123