Interleukin-2 as a marker for detecting asymptomatic individuals in areas where Leishmania infantum is endemic

被引:29
作者
Ibarra-Meneses, A. V. [1 ]
Carrillo, E. [1 ]
Sanchez, C. [1 ]
Garcia-Martinez, J. [2 ]
Lopez Lacomba, D. [2 ]
San Martin, J. V. [3 ]
Alves, F. [4 ]
Alvar, J. [4 ]
Moreno, J. [1 ]
机构
[1] Inst Salud Carlos III, WHO Collaborating Ctr Leishmaniasis, Ctr Nacl Microbiol, Madrid, Spain
[2] Hosp Univ Fuenlabrada, Lab Med, Blood Bank & Haemotherapy Dept, Madrid, Spain
[3] Hosp Univ Fuenlabrada, Dept Infect Dis, Internal Med, Madrid, Spain
[4] DNDi, Visceral Leishmaniasis Program, Geneva, Switzerland
关键词
Asymptomatic; Interferon-gamma release assay; Interleukin-2; Leishmaniasis; Marker; VISCERAL LEISHMANIASIS; ACTIVE TUBERCULOSIS; WHOLE-BLOOD; IFN-GAMMA; SKIN-TEST; INFECTION; ASSAY; QUANTIFERON; RESPONSES; EXPOSURE;
D O I
10.1016/j.cmi.2016.05.021
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
No field method exists for identifying asymptomatic individuals in areas where Leishmania infantum is endemic. This work reports that, 24 h after stimulating whole blood with soluble Leishmania antigen (SLA), plasma interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) become significantly elevated in samples from asymptomatic individuals (n = 47) compared with those from negative controls (n = 50), all of them recruited from a blood bank. When compared with the reference test SLA-lymphoproliferative assay, IL-2 appears as a new, 100% sensitive and specific marker for asymptomatic individuals with a positive cellular response (compared with 100% and 84.78%, respectively, for IFN-gamma). Further studies in other transmission areas and in other cohorts of exposed people need to be performed to confirm these results. Once validated, IFN-gamma and IL-2 levels in SLA-stimulated whole blood could be reliably used in the field to estimate the prevalence of those asymptomatic individuals with Leishmania-specific cellular immune responses. A.V. Ibarra-Meneses, (C) 2016 The Author(s). Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:739.e1 / 739.e4
页数:4
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