A new cytochrome P450 system from Bacillus megaterium DSM319 for the hydroxylation of 11-keto-β-boswellic acid (KBA)

被引:33
作者
Brill, Elisa [1 ]
Hannemann, Frank [1 ]
Zapp, Josef [2 ]
Bruening, Gerit [3 ]
Jauch, Johann [3 ]
Bernhardt, Rita [1 ,4 ]
机构
[1] Univ Saarland, Dept Biochem, D-66123 Saarbrucken, Germany
[2] Univ Saarland, Dept Pharmaceut Biol, D-66123 Saarbrucken, Germany
[3] Univ Saarland, Dept Organ Chem 2, D-66123 Saarbrucken, Germany
[4] Univ Saarland, Inst Biochem, D-66123 Saarbrucken, Germany
关键词
Cytochromes P450; CYP106A1; Flavodoxin; Ferredoxin; Bacillus megaterium; 11-Keto-beta-boswellic acid; ACETYL-BOSWELLIC ACIDS; SYNTHETIC APPLICATION; FERREDOXIN ACTIVITY; ESCHERICHIA-COLI; EXPRESSION; PROTEINS; FLAVODOXIN; BINDING; IDENTIFICATION; REDUCTASE;
D O I
10.1007/s00253-013-5029-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In the genome of Bacillus megaterium DSM319, a strain who has recently been sequenced to fully exploit its potential for biotechnological purposes, we identified a gene encoding the cytochrome P450 CYP106A1 as well as genes encoding potential redox partners of CYP106A1. We cloned, expressed, and purified CYP106A1 and five potential autologous redox partners, one flavodoxin and four ferredoxins. The flavodoxin and three ferredoxins were able to support the activity of CYP106A1 displaying the first cloned natural redox partners of a cytochrome P450 from B. megaterium. The CYP106A1 system was able to convert the pentacyclic triterpene 11-keto-beta-boswellic acid (KBA) belonging to the main bioactive constituents of Boswellia serrata gum resin extracts, which are used to treat inflammatory disorders and arthritic diseases. In order to provide sufficient amounts of the KBA products to characterize them structurally by NMR spectroscopy, recombinant whole-cell biocatalysts were constructed based on B. megaterium MS941. The main product has been identified as 7 beta-hydroxy-KBA, while the side product (similar to 20 %) was shown to be a mixture of 7 beta, 15 alpha-dihydroxy-KBA and 15 alpha-hydroxy-KBA. Without further optimization 560.7 mg l(-1) day(-1) of the main product, 7 beta-hydroxy-KBA, could be obtained thus providing a suitable starting point for the efficient production of modified KBA by chemical tailoring to produce novel KBA derivatives with increased bioavailability and this way more efficient drugs.
引用
收藏
页码:1703 / 1717
页数:15
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