OsCKX2 regulates phosphate deficiency tolerance by modulating cytokinin in rice

被引:13
作者
Yan, Huimin [1 ]
Wang, Yale [1 ]
Chen, Bo [1 ]
Wang, Weijie [1 ]
Sun, Hongzheng [1 ]
Sun, Huwei [1 ]
Li, Junzhou [1 ]
Zhao, Quanzhi [1 ]
机构
[1] Henan Agr Univ, Collaborat Innovat Ctr Henan Grain Crops, Henan Key Lab Rice Biol, Zhengzhou 450002, Peoples R China
关键词
OsCKX2; Phosphate deficiency; Transcriptome; Metabolome; Rice; STARVATION RESPONSE; AFFECTS GROWTH; ARABIDOPSIS; PLANT; AUXIN; GENE; TRANSPORTERS; OVEREXPRESSION; ARCHITECTURE; ADAPTATIONS;
D O I
10.1016/j.plantsci.2022.111257
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cytokinin oxidase/dehydrogenases (CKXs) are key enzymes that degrade cytokinins (CTKs) and play an essential role in plant growth and development. The present study analyzed the phenotypic and physiological charac-teristics of OsCKX2 overexpressing (OE) and knockout (KO) rice plants after exposure to phosphate (Pi) defi-ciency and the transcriptome and metabolome to investigate the function of OsCKX2 in response to Pi deficiency. OsCKX2 KO plants demonstrated higher endogenous CTK levels than wild-type (WT) under Pi deficiency. Further analysis indicated more robust tolerance of OsCKX2 KO plants to Pi deficiency, which exhibited higher phos-phorus concentration, larger shoot biomass, and lesser leaf yellowing under Pi deficiency; whereas the opposite was observed for OsCKX2 OE plants. Transcriptome and metabolome analyses revealed that overexpression of OsCKX2 downregulated the transcriptional levels of genes related to Pi transporters, membrane lipid metabolism, and glycolysis, and reduced the consumption of metabolites in membrane lipid metabolism and glycolysis. On the contrary, knockout of OsCKX2 upregulated the expression of Pi transporters, and increased the consumption of metabolites in membrane lipid metabolism and glycolysis. These results indicated that OsCKX2 impacted Pi uptake, recycling, and plant growth via Pi transporters, phospholipid hydrolysis, and glycolysis under Pi defi-ciency. Overall, OsCKX2 negatively regulated Pi deficiency tolerance by modulating CTKs in rice.
引用
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页数:12
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