Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus

被引：65

作者：

Deng, Kangyu ^{[1
]}

Pan, Mingzhu ^{[1
]}

Liu, Jiahuan ^{[1
]}

MengxiYang ^{[1
]}

Gu, Zhixiang ^{[1
]}

Zhang, Yue ^{[1
]}

Liu, Guangxia ^{[1
]}

Liu, Dong ^{[1
]}

Zhang, Wenbing ^{[1
,2
]}

Mai, Kangsen ^{[1
,2
]}

机构：

[1] Ocean Univ China, Key Lab Mariculture, Minist Educ, Minist Agr,Key Lab Aquaculture Nutr & Feeds, Qingdao 266003, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Wen Hai Rd, Qingdao 266237, Peoples R China

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

中国国家自然科学基金;

关键词：

TROUT ONCORHYNCHUS-MYKISS; PLASMA-INSULIN LEVELS; CARP CYPRINUS-CARPIO; RAINBOW-TROUT; GENE-EXPRESSION; FEED-UTILIZATION; OXIDATIVE STRESS; BODY-COMPOSITION; TNF-ALPHA; NUTRITIONAL REGULATION;

D O I：

10.1038/s41598-018-25412-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Carnivorous fish is thought to be high-glucose intolerance. But the reasons were still unclear. The aim of the present study is to investigate the effects of high level of dietary carbohydrate on the survival, growth and immune responses of Paralichthys olivaceus, and the underlying molecular mechanism related to the immune and glucose metabolism. P. olivaceus were fed with 8%, 16% and 24% of dietary carbohydrate for 10 weeks, respectively. After that, a glucose tolerance test (GTT) was conducted. Results showed that excessive (24%) dietary carbohydrate significantly decreased the growth and glucose tolerance ability according to the GTT. It significantly increased hepatic NADPH oxidase activity and malondialdehyde content and serum contents of IL-6 and advanced glycation end products. The expressions of glucose transport-relevant genes in liver and the content of related hormones in serum were analyzed. In conclusion, it was confirmed that IL-6 increased the expression of suppressor of cytokine signaling 3 (SOCS3) and regulated the downstream targets of PI3K-AKT mediated signal transduction, and then downregulated the glucose transporter 2 activity in liver of P. olivaceus fed diet with excessive carbohydrate level. It was suggested that SOCS3 served as a bridge between immune response and glucose metabolism in P. olivaceus.

引用

页数：13

共 91 条

[1] The c-Jun NH2-terminal kinase promotes insulin resistance during association with insulin receptor substrate-1 and phosphorylation of Ser307 [J].