Development and Evaluation of a Blocking Enzyme-Linked Immunosorbent Assay and Virus Neutralization Assay To Detect Antibodies to Viral Hemorrhagic Septicemia Virus

被引：16
|
作者：
Wilson, Anna [1 ]
Goldberg, Tony [1 ]
Marcquenski, Susan [2 ]
Olson, Wendy [3 ]
Goetz, Frederick [4 ]
Hershberger, Paul [5 ]
Hart, Lucas [5 ]
Toohey-Kurth, Kathy [1 ,6 ]
机构：
[1] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA
[2] Wisconsin Dept Nat Resources, Bur Fisheries Management, Madison, WI USA
[3] Med Coll Wisconsin, Dept Pathol, Milwaukee, WI 53226 USA
[4] NOAA, Manchester Res Stn, Port Orchard, WA USA
[5] US Geol Survey, Western Fisheries Res Ctr, Marrowstone Marine Field Stn, Nordland, WA USA
[6] Univ Wisconsin, Wisconsin Vet Diagnost Lab, Madison, WI 53706 USA
来源：
CLINICAL AND VACCINE IMMUNOLOGY | 2014年 / 21卷 / 03期
基金：
美国海洋和大气管理局;
关键词：
PERCA-FLAVESCENS; YELLOW PERCH; EGTVED VIRUS; COMPARATIVE SUSCEPTIBILITY; SPRING VIREMIA; LAKE-ONTARIO; CARP VIRUS; VHSV; AMERICAN; WILD;
D O I：
10.1128/CVI.00675-13
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Viral hemorrhagic septicemia virus (VHSV) is a target of surveillance by many state and federal agencies in the United States. Currently, the detection of VHSV relies on virus isolation, which is lethal to fish and indicates only the current infection status. A serological method is required to ascertain prior exposure. Here, we report two serologic tests for VHSV that are nonlethal, rapid, and species independent, a virus neutralization (VN) assay and a blocking enzyme-linked immunosorbent assay (ELISA). The results show that the VN assay had a specificity of 100% and sensitivity of 42.9%; the anti-nucleocapsid-blocking ELISA detected nonneutralizing VHSV antibodies at a specificity of 88.2% and a sensitivity of 96.4%. The VN assay and ELISA are valuable tools for assessing exposure to VHSV.
引用
收藏
页码：435 / 442
页数：8
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