Synthesis and Polymerase Chain Reaction Amplification of DNA Strands Containing an Unnatural Triazole Linkage

被引：92

作者：

El-Sagheer, Afaf H. ^{[1
,2
]}

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Suez Canal Univ, Fac Petr & Min Engn, Dept Sci & Math, Suez, Egypt

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 11期

关键词：

LIGATION; ACID;

D O I：

10.1021/ja8065896

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleoticles with T-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplicons and clones in two different sequence contexts revealed the presence of a single thymidine at the ligation site. The remarkable ability of thermostable polymerases to reproducibly copy DNA templates containing such an unnatural backbone opens up intriguing possibilities in gene synthesis, genetic analysis, biology, and nanotechnology.

引用

页码：3958 / 3964

页数：7