The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens

被引：13

作者：

Drews, Steven J. ^{[1
,2
]}

Eshaghi, AliReza ^{[1
]}

Pyskir, Daria ^{[1
]}

Chedore, Pam ^{[1
]}

Lombos, Ernesto ^{[1
]}

Broukhanski, George ^{[1
]}

Higgins, Rachel ^{[1
]}

Fisman, David N. ^{[1
,3
]}

Blair, Joanne ^{[1
]}

Jamieson, Frances ^{[1
,4
]}

机构：

[1] Minist Hlth & Long Term Care, Publ Hlth Labs Branch, Toronto, ON, Canada

[2] Mt Sinai Hosp, Dept Microbiol, Toronto, ON M5G 1X5, Canada

[3] Hosp Sick Children, Toronto, ON M5G 1X8, Canada

[4] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

来源：

DIAGNOSTIC PATHOLOGY | 2008年 / 3卷 / 1期

关键词：

D O I：

10.1186/1746-1596-3-37

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The Seeplex (TM) TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex ( MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.

引用

页数：4

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