Effect of Baicalein on Matrix Metalloproteinases and Durability of Resin-Dentin Bonding

被引:20
作者
Li, J. [1 ,2 ]
Chen, B. [1 ,2 ]
Hong, N. [1 ,2 ]
Wu, S. [1 ,2 ]
Li, Y. [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Dept Prosthodont, Guangzhou, Guangdong, Peoples R China
[2] Guangdong Prov Key Lab Stomatol, Guangzhou, Guangdong, Peoples R China
关键词
ISCHEMIA-REPERFUSION INJURY; CLINICALLY RELEVANT TIME; DIMETHYL-SULFOXIDE; CROSS-LINKING; RINSE ADHESIVES; IN-VITRO; MECHANICAL-PROPERTIES; SIGNALING PATHWAY; CANCER CELLS; DEGRADATION;
D O I
10.2341/17-097-L
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective: In an attempt to increase resindentin bonding quality, this study used baicalein as a preconditioner in an etch-and-rinse adhesive to evaluate its effect on matrix metalloproteinases (MMPs) and adhesive durability. Methods: As a MMP inhibitor and potential collagen cross-linking agent, baicalein was used as a preconditioner in an etch-and-rinse adhesive system. The degree of conversion was evaluated by Fourier-transform infrared spectroscopy. EnzChek gelatinase/collagenase assay kits were then used to detect the MMP inhibitory effect of different concentrations of baicalein (0.1, 0.5, 2.5, and 5.0 mu g/mL) on dentin powders. During in vitro bonding procedures, flat dentin surfaces on sound third molars were preconditioned with 2.5 mu g/mL baicalein after being acid-etched; this step was followed by continuation of adhesive processes and build-up of resin composite. After resin-dentin stick preparation, bonding strength, failure mode, and interface nanoleakage were respectively evaluated via microtensile testing, stereomicroscopy, and field emission scanning electron microscopy either immediately or after storage in artificial saliva for three or six months. Data were analyzed by two-way analysis of variance and Tukey test (alpha=0.05). Results: Baicalein at a concentration of 0-5.0 mu g/mL did not influence the conversion of adhesives. However, it inhibited the activities of dentin-bond gelatinase and collagenase, especially at a concentration of 2.5 mu g/mL, while effectively increasing microtensile bonding strength and decreasing nanoleakage in vitro, both immediately and after aging. Conclusions: Baicalein used as preconditioner in an etch-and-rinse adhesive system has an anti-MMP function and effectively improves resindentin bonding durability in vitro, which has potential value in clinical bonding procedures.
引用
收藏
页码:426 / 436
页数:11
相关论文
共 53 条
[1]   Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury [J].
Akhlaghi, Masoumeh ;
Bandy, Brian .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2009, 46 (03) :309-317
[2]   Effect of Dentin Pretreatment on Bond Strength Stability of Self-etching and Etch-and-Rinse Adhesives to Intracoronally Bleached Dentin [J].
Borges de Souza, Ilzes Marianne ;
Araujo, Camila Silva ;
Soares, Carlos Jose ;
Faria-e-Silva, Andre Luis .
JOURNAL OF ADHESIVE DENTISTRY, 2016, 18 (04) :349-354
[3]   Gelatinases in soft tissue biomaterials. Analysis of different crosslinking agents [J].
Calero, P ;
Jorge-Herrero, E ;
Turnay, J ;
Olmo, N ;
de Silanes, IL ;
Lizarbe, MA ;
Maestro, MM ;
Arenaz, B ;
Castillo-Olivares, JL .
BIOMATERIALS, 2002, 23 (16) :3473-3478
[4]   Mechanical characterization of proanthocyanidindentin matrix interaction [J].
Castellan, Carina Strano ;
Pereira, Patricia Nobrega ;
Miranda Grande, Rosa Helena ;
Bedran-Russo, Karina .
DENTAL MATERIALS, 2010, 26 (10) :968-973
[5]   Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes [J].
Chang, Wei-Tien ;
Shao, Zuo-Hui ;
Yin, Jun-Jie ;
Mehendale, Sangeeta ;
Wang, Chong-Zhi ;
Qin, Yimin ;
Li, Juan ;
Chen, Wen-Jone ;
Chien, Chiang-Ting ;
Becker, Lance B. ;
Hoek, Terry L. Vanden ;
Yuan, Chun-Su .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2007, 566 (1-3) :58-66
[6]   Baicalein Inhibits the Invasion and Metastatic Capabilities of Hepatocellular Carcinoma Cells via Down-Regulation of the ERK Pathway [J].
Chen, Kunlun ;
Zhang, Shu ;
Ji, Yuanyuan ;
Li, Jun ;
An, Peng ;
Ren, Hongtao ;
Liang, Rongrui ;
Yang, Jun ;
Li, Zongfang .
PLOS ONE, 2013, 8 (09)
[7]   Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes [J].
Chen, Wei-Ping ;
Xiong, Yan ;
Hu, Peng-Fei ;
Bao, Jia-Peng ;
Wu, Li-Dong .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2015, 36 (01) :325-333
[8]  
Choi EO, 2016, BIOMOLECULAR THERAPE, V25, P213
[9]   Influence of glutaraldehyde priming on bond strength of an experimental adhesive system applied to wet and dry dentine [J].
Cilli, Renato ;
Prakki, Anuradha ;
de Araujo, Paulo Amarante ;
Pereira, Jose Carlos .
JOURNAL OF DENTISTRY, 2009, 37 (03) :212-218
[10]   Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives [J].
De Munck, Jan ;
Mine, Atsushi ;
Van den Steen, Philippe E. ;
Van Landuyt, Kirsten L. ;
Poitevin, Andre ;
Opdenakker, Ghislain ;
Van Meerbeek, Bart .
EUROPEAN JOURNAL OF ORAL SCIENCES, 2010, 118 (05) :494-501