Sensitive and non-separation 5-hydroxymethylcytosine double-stranded DNA sensing in different mice tissues: Electrogenerated chemiluminescence biosensing method incorporating DNA nanomachine amplification and nanocomposite magnetic separation

被引：3

作者：

Li, Sijia ^{[1
]}

Zhang, Shengtao ^{[1
]}

Shi, Jiayue ^{[1
]}

Yang, Xia ^{[1
]}

Sun, Huiping ^{[2
]}

Li, Yan ^{[1
]}

机构：

[1] Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710069, Peoples R China

[2] Suzhou Univ Sci & Technol, Inst Mat Sci & Devices, Suzhou 215000, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2022年 / 368卷

基金：

中国国家自然科学基金;

关键词：

Electrogenerated chemiluminescence; 5-Hydroxymethylcytosine; DNA nanomachine; Nanocomposite magnetic separation; Mice tissue; NANOPARTICLES;

D O I：

10.1016/j.snb.2022.132179

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, a sensitive and non-separation electrogenerated chemiluminescence (ECL) biosensing method that combines poly (diallyl dimethyl ammonium chloride)/ferrosoferric oxide/molybdenum disulfide (PDDA/Fe3O4/ MoS2) nanocomposite with DNA nanomachine was developed to determine 5-hydroxymethylcytosine modified double-strand DNA (5-hmC-dsDNA). A positively charged and magnetic PDDA/Fe3O4/MoS2 nanocomposite was proposed, which can be used to fix negatively charged 5-hmC-dsDNA to overcome the steric resistance problem and is available for magnetic fields separation. In addition, the DNA nanomachine with three elements was fabricated: a DNA walking probe for signal amplification, an ECL reagent labeled S1 DNA (Ru-S1), 5-hmC antibody (Ab) for specifically recognizing 5-hmC modification. In the presence of 5-hmC-dsDNA, sandwich type conjugates were generated to incubate PDDA/Fe3O4/MoS2 and DNA nanomachine. After magnetic separation and the DNA walking driven by enzyme cleavage, a lot of ECL reagents were released and increased the ECL signal. ECL measurement was performed in a separation-free format. The method showed a broader linear range (0.0090-0.5761 %) and a low limit of detection (0.0024 %). Furthermore, the determination of 5-hmCdsDNA in different mice tissues using the ECL biosensing method was also carried out, demonstrating that the established strategy may pave a novel avenue for the early clinical diagnosis of disease.

引用

页数：8